PMID- 23313572
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20211021
IS  - 1089-8611 (Electronic)
IS  - 1089-8603 (Print)
IS  - 1089-8603 (Linking)
VI  - 30
DP  - 2013 Apr 1
TI  - Hemoglobin infusion does not alter murine pulmonary vascular tone.
PG  - 1-8
LID - S1089-8603(13)00003-7 [pii]
LID - 10.1016/j.niox.2012.12.007 [doi]
AB  - Plasma hemoglobin (Hb) scavenges endothelium-derived nitric oxide (NO), producing 
      systemic and pulmonary vasoconstriction in many species. We hypothesized that 
      i.v. administration of murine cell-free Hb would produce pulmonary 
      vasoconstriction and enhance hypoxic pulmonary vasoconstriction (HPV) in mice. To 
      assess the impact of plasma Hb on basal pulmonary vascular tone in anesthetized 
      mice we measured left lung pulmonary vascular resistance (LPVRI) before and after 
      infusion of Hb at thoracotomy. To confirm the findings obtained at thoracotomy, 
      measurements of right ventricular systolic pressure (RVSP) and systemic arterial 
      pressure (SAP) were obtained in closed-chest wild-type mice. To elucidate whether 
      pretreatment with Hb augments HPV we assessed the increase in LPVRI before and 
      during regional lung hypoxia produced by left mainstem bronchial occlusion (LMBO) 
      in wild-type mice pretreated with Hb. Infusion of Hb increased SAP but did not 
      change pulmonary arterial pressure (PAP), left lung pulmonary arterial flow 
      (QLPA) or LPVRI in either wild-type or diabetic mice with endothelial 
      dysfunction. Scavenging of NO by plasma Hb did not alter HPV in wild-type mice. 
      Inhibition of NO synthase with l-NAME did not change the basal LPVRI, but 
      augmented HPV during LMBO. Our data suggest that scavenging of NO by plasma Hb 
      does not alter pulmonary vascular tone in mice. Therefore, generation of NO in 
      the pulmonary circulation is unlikely to be responsible for the low basal 
      pulmonary vascular tone of mice.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Beloiartsev, Arkadi
AU  - Beloiartsev A
AD  - Postdoctoral Fellow, Anesthesia Center for Critical Care Research of the 
      Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General 
      Hospital and Harvard Medical School, Boston, MA 02114-2696, USA.
FAU - Baron, David M
AU  - Baron DM
FAU - Yu, Binglan
AU  - Yu B
FAU - Bloch, Kenneth D
AU  - Bloch KD
FAU - Zapol, Warren M
AU  - Zapol WM
LA  - eng
GR  - R01 HL074352/HL/NHLBI NIH HHS/United States
GR  - HL074352/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130108
PL  - United States
TA  - Nitric Oxide
JT  - Nitric oxide : biology and chemistry
JID - 9709307
RN  - 0 (Hemoglobins)
RN  - 0 (Vasoconstrictor Agents)
RN  - 11062-77-4 (Superoxides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 76898-47-0 (15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid/pharmacology
MH  - Analysis of Variance
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Diabetes Mellitus, Experimental
MH  - Hemoglobins/*administration & dosage
MH  - Hypoxia
MH  - Lung/*blood supply/chemistry/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NG-Nitroarginine Methyl Ester/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/metabolism
MH  - Superoxides/metabolism
MH  - Thoracotomy
MH  - Vascular Resistance/*drug effects
MH  - Vasoconstriction/*drug effects
MH  - Vasoconstrictor Agents/pharmacology
PMC - PMC3764648
MID - NIHMS504089
EDAT- 2013/01/15 06:00
MHDA- 2013/09/24 06:00
PMCR- 2014/04/01
CRDT- 2013/01/15 06:00
PHST- 2012/11/21 00:00 [received]
PHST- 2012/12/18 00:00 [revised]
PHST- 2012/12/28 00:00 [accepted]
PHST- 2013/01/15 06:00 [entrez]
PHST- 2013/01/15 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - S1089-8603(13)00003-7 [pii]
AID - 10.1016/j.niox.2012.12.007 [doi]
PST - ppublish
SO  - Nitric Oxide. 2013 Apr 1;30:1-8. doi: 10.1016/j.niox.2012.12.007. Epub 2013 Jan 
      8.