PMID- 23313881
OWN - NLM
STAT- MEDLINE
DCOM- 20130821
LR  - 20130304
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 43
DP  - 2013 May 15
TI  - Label-free electrochemical IgE aptasensor based on covalent attachment of aptamer 
      onto multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite modified 
      electrode.
PG  - 218-25
LID - S0956-5663(12)00858-5 [pii]
LID - 10.1016/j.bios.2012.12.006 [doi]
AB  - A simple, sensitive and label-free aptamer-based biosensor for the detection of 
      human immunoglobulin E (IgE) is developed using the electrochemical transduction 
      method. A special immobilization interface consisting of multiwalled carbon 
      nanotubes/ionic liquid/chitosan nanocomposite (MWCNTs/IL/Chit) is utilized to 
      improve the conductivity and performance characteristics of the biosensor as well 
      as to increase the loading amount of aptamer DNA sequence. A 5'-amino-terminated 
      aptamer is covalently attached onto MWCNTs/IL/Chit modified glassy carbon (GC) 
      electrode via a linker of glutaraldehyde (GA). Methylene blue (MB) is used as an 
      electrochemical indicator which is intercalated into the aptamer through the 
      specific interaction with its guanine bases. In the absence of IgE, MB bound to 
      the aptamer produces a strong differential pulse voltammetric (DPV) signal. But 
      when IgE exists, the intercalated MB releases from the aptamer, resulting an 
      obviously decreased DPV signal. This phenomenon can be applied for human IgE 
      detection. The peak current of MB linearly decreases with the concentration of 
      IgE over a range of 0.5-30 nM with a detection limit of 37 pM. By using Bovine 
      serum albumin (BSA) and lysozyme, the excellent specificity of this sensing 
      system for the detection of IgE is also demonstrated. Finally, the proposed 
      aptasensor is successfully used to IgE analysis in human serum sample. The 
      obtained result is well agreed with the value obtained by the standard ELISA 
      method. The herein described approach is expected to promote the exploitation of 
      aptamer-based biosensors for protein assays in biochemical and biomedical 
      studies.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Khezrian, Somayeh
AU  - Khezrian S
AD  - Department of Chemistry, University of Kurdistan, P. O. Box 416, Sanandaj, Iran.
FAU - Salimi, Abdollah
AU  - Salimi A
FAU - Teymourian, Hazhir
AU  - Teymourian H
FAU - Hallaj, Rahman
AU  - Hallaj R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121220
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Ionic Liquids)
RN  - 0 (Nanotubes, Carbon)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/*instrumentation
MH  - Chitosan/*chemistry
MH  - Conductometry/*instrumentation
MH  - *Electrodes
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Immunoglobulin E/*blood
MH  - Ionic Liquids/*chemistry
MH  - Nanotubes, Carbon/*chemistry/ultrastructure
MH  - Staining and Labeling
EDAT- 2013/01/15 06:00
MHDA- 2013/08/22 06:00
CRDT- 2013/01/15 06:00
PHST- 2012/06/14 00:00 [received]
PHST- 2012/11/25 00:00 [revised]
PHST- 2012/12/04 00:00 [accepted]
PHST- 2013/01/15 06:00 [entrez]
PHST- 2013/01/15 06:00 [pubmed]
PHST- 2013/08/22 06:00 [medline]
AID - S0956-5663(12)00858-5 [pii]
AID - 10.1016/j.bios.2012.12.006 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 May 15;43:218-25. doi: 10.1016/j.bios.2012.12.006. Epub 
      2012 Dec 20.