PMID- 23314487
OWN - NLM
STAT- MEDLINE
DCOM- 20130814
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 405
IP  - 8
DP  - 2013 Mar
TI  - Anionic metabolic profiling of urine from antibiotic-treated rats by capillary 
      electrophoresis-mass spectrometry.
PG  - 2585-94
LID - 10.1007/s00216-012-6701-4 [doi]
AB  - A recently developed capillary electrophoresis (CE)-negative-ionisation mass 
      spectrometry (MS) method was used to profile anionic metabolites in a 
      microbial-host co-metabolism study. Urine samples from rats receiving antibiotics 
      (penicillin G and streptomycin sulfate) for 0, 4, or 8 days were analysed. A 
      quality control sample was measured repeatedly to monitor the performance of the 
      applied CE-MS method. After peak alignment, relative standard deviations (RSDs) 
      for migration time of five representative compounds were below 0.4 %, whereas 
      RSDs for peak area were 7.9-13.5 %. Using univariate and principal component 
      analysis of obtained urinary metabolic profiles, groups of rats receiving 
      different antibiotic treatment could be distinguished based on 17 discriminatory 
      compounds, of which 15 were downregulated and 2 were upregulated upon treatment. 
      Eleven compounds remained down- or upregulated after discontinuation of the 
      antibiotics administration, whereas a recovery effect was observed for others. 
      Based on accurate mass, nine compounds were putatively identified; these included 
      the microbial-mammalian co-metabolites hippuric acid and indoxyl sulfate. Some 
      discriminatory compounds were also observed by other analytical techniques, but 
      CE-MS uniquely revealed ten metabolites modulated by antibiotic exposure, 
      including aconitic acid and an oxocholic acid. This clearly demonstrates the 
      added value of CE-MS for nontargeted profiling of small anionic metabolites in 
      biological samples.
FAU - Kok, Miranda G M
AU  - Kok MG
AD  - Biomolecular Analysis, Department of Pharmaceutical Sciences, Utrecht University, 
      Utrecht, The Netherlands. M.G.M.Kok@uu.nl
FAU - Ruijken, Marco M A
AU  - Ruijken MM
FAU - Swann, Jonathan R
AU  - Swann JR
FAU - Wilson, Ian D
AU  - Wilson ID
FAU - Somsen, Govert W
AU  - Somsen GW
FAU - de Jong, Gerhardus J
AU  - de Jong GJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20130112
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Anti-Bacterial Agents)
RN  - Q42T66VG0C (Penicillin G)
RN  - Y45QSO73OB (Streptomycin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*metabolism/*urine
MH  - Electrophoresis, Capillary/*methods
MH  - Mass Spectrometry/*methods
MH  - *Metabolome
MH  - Penicillin G/metabolism/urine
MH  - Rats
MH  - Streptomycin/metabolism/urine
EDAT- 2013/01/15 06:00
MHDA- 2013/08/15 06:00
CRDT- 2013/01/15 06:00
PHST- 2012/11/02 00:00 [received]
PHST- 2012/12/22 00:00 [accepted]
PHST- 2012/12/19 00:00 [revised]
PHST- 2013/01/15 06:00 [entrez]
PHST- 2013/01/15 06:00 [pubmed]
PHST- 2013/08/15 06:00 [medline]
AID - 10.1007/s00216-012-6701-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2013 Mar;405(8):2585-94. doi: 10.1007/s00216-012-6701-4. Epub 
      2013 Jan 12.