PMID- 23315148
OWN - NLM
STAT- MEDLINE
DCOM- 20140825
LR  - 20140110
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 52
IP  - 2
DP  - 2014 Feb
TI  - Simultaneous screening and identifying four categories of particular flavonoids 
      in the leaves of Murraya exotica L. by HPLC-DAD-ESI-MS-MS.
PG  - 103-14
LID - 10.1093/chromsci/bms253 [doi]
AB  - Polymethoxylated flavonoids (PMFs), the particular flavonoid subclass in which 
      all or almost all hydroxyls are capped by methylation, have high oral 
      bioavailability and various activities. A sensitive high-performance liquid 
      chromatography-diode array detection-electrospray ionization tandem mass 
      spectrometry (HPLC-DAD-ESI-MS-MS) method was established to screen and identify 
      the PMFs in the leaves of Murraya exotica. Eight PMF standards, including two 
      polymethoxylated flavones, two polymethoxylated flavanones, two polymethoxylated 
      chalcones and two PMF glycosides, were first to be analyzed in positive mode by 
      collision-induced dissociation MS-MS. On the basis of the ESI-MS, 
      characterizations were deduced, and in the results of the extracted ion 
      chromatogram MS-MS experiment, 26 PMFs, including 18 flavones, five flavanones or 
      chalcones and three PMF glycosides, were screened out from the complex extract of 
      the leaves of M. exotica. Among them, 24 PMFs were hydroxylated 
      polymethoxyflavonoids, whereas the rest were all permethoxylated PMFs. This was 
      the first systematic report on the presence of PMFs in the leaves of M. exotica. 
      The results indicated that the established analytical method could be adopted as 
      a rapid, effective technique for the structural characterization of PMFs from the 
      complex extracts of traditional Chinese medicines.
FAU - Zhang, Jiayu
AU  - Zhang J
AD  - 1School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 
      100102, China.
FAU - Lu, Jianqiu
AU  - Lu J
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Dai, Long
AU  - Dai L
FAU - Liu, Ying
AU  - Liu Y
FAU - Tu, Pengfei
AU  - Tu P
FAU - Qiao, Yanjiang
AU  - Qiao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130111
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Flavonoids/*analysis/chemistry
MH  - Murraya/*chemistry
MH  - Plant Extracts/*chemistry
MH  - Plant Leaves/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/01/15 06:00
MHDA- 2014/08/26 06:00
CRDT- 2013/01/15 06:00
PHST- 2013/01/15 06:00 [entrez]
PHST- 2013/01/15 06:00 [pubmed]
PHST- 2014/08/26 06:00 [medline]
AID - bms253 [pii]
AID - 10.1093/chromsci/bms253 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2014 Feb;52(2):103-14. doi: 10.1093/chromsci/bms253. Epub 2013 
      Jan 11.