PMID- 23317849
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20131031
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 137
IP  - 1-2
DP  - 2013 Feb
TI  - Effect of the stage of estrous cycle on follicular population, oocyte yield and 
      quality, and biochemical composition of serum and follicular fluid in Anatolian 
      water buffalo.
PG  - 8-14
LID - S0378-4320(12)00402-2 [pii]
LID - 10.1016/j.anireprosci.2012.12.004 [doi]
AB  - This study was designed to investigate the effects that the different stages of 
      the estrous cycle had on the number of surface ovarian follicles and oocyte yield 
      and quality of Anatolian water buffalo during peak breeding season. Assessments 
      were made on the basis of ovarian morphology, serum and follicular fluid 
      concentrations of variety of biochemical parameters. Following slaughter, blood 
      samples were collected from each animal. The stage of estrous cycle was 
      classified as either the luteal or follicular phase, and surface ovarian 
      follicles were classified as small, medium, or large. The follicular fluid was 
      aspirated, and oocytes were evaluated microscopically for classification into 
      four categories. No statistical differences (p>0.05) were observed regarding the 
      total number of follicles or quality of oocytes relative to the stage of the 
      estrous cycle. Serum high-density lipoprotein cholesterol (HDL), alkaline 
      phosphatase (ALP) and progesterone (P4) concentrations were significantly higher 
      in the luteal phase than in the follicular phase (P<0.05). Significant 
      correlations were observed in the luteal phase between the total number of 
      oocytes and cholesterol (Cho), HDL, sodium (Na), chloride (Cl); A-quality oocytes 
      and Na, Cl, Mg; C-quality oocytes and Cho, HDL, and Mg in follicular fluid. These 
      results offer new information concerning Anatolian water buffalo reproductive 
      physiology, which may be useful for improving oocyte quality in buffalo. This is 
      the first study to describe the number of ovarian follicles, oocyte yield and 
      quality, and a variety of biochemical parameters in the serum and follicular 
      fluid of Anatolian water buffalo during peak breeding season in Turkey.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Baki Acar, Duygu
AU  - Baki Acar D
AD  - Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Afyon 
      Kocatepe University, Afyonkarahisar, Turkey. Electronic address: 
      dbakiacar@gmail.com.
FAU - Birdane, Muhammed Kursad
AU  - Birdane MK
FAU - Dogan, Nurhan
AU  - Dogan N
FAU - Gurler, Hande
AU  - Gurler H
LA  - eng
PT  - Journal Article
DEP - 20130102
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/blood
MH  - Animals
MH  - Buffaloes/blood/*physiology
MH  - Cholesterol/blood
MH  - Estrous Cycle/blood/*physiology
MH  - Female
MH  - Oocytes/physiology/ultrastructure
MH  - Ovarian Follicle/cytology/*physiology
MH  - Progesterone/blood
MH  - Seasons
MH  - Statistics, Nonparametric
MH  - Turkey
EDAT- 2013/01/16 06:00
MHDA- 2014/06/04 06:00
CRDT- 2013/01/16 06:00
PHST- 2012/05/09 00:00 [received]
PHST- 2012/12/10 00:00 [revised]
PHST- 2012/12/12 00:00 [accepted]
PHST- 2013/01/16 06:00 [entrez]
PHST- 2013/01/16 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
AID - S0378-4320(12)00402-2 [pii]
AID - 10.1016/j.anireprosci.2012.12.004 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2013 Feb;137(1-2):8-14. doi: 10.1016/j.anireprosci.2012.12.004. 
      Epub 2013 Jan 2.