PMID- 23319191
OWN - NLM
STAT- MEDLINE
DCOM- 20131113
LR  - 20191112
IS  - 1509-572X (Electronic)
IS  - 1509-572X (Linking)
VI  - 50
IP  - 4
DP  - 2012
TI  - CD133/CD15 defines distinct cell subpopulations with differential in vitro 
      clonogenic activity and stem cell-related gene expression profile in in vitro 
      propagated glioblastoma multiforme-derived cell line with a PNET-like component.
PG  - 357-68
AB  - Glioblastoma multiforme (GBM), as many other solid tumours, contains a 
      subpopulation of cells termed cancer stem-like cells responsible for the 
      initiation and propagation of tumour growth. However, a unique 
      immunophenotype/surface antigen composition for the clear identification of brain 
      tumour stem cells (BTSC) has not yet been found. Here we report a novel code of 
      cell surface markers for the identification of different cell subpopulations in 
      neurospheres derived from a GBM with a primitive neuroectodermal tumour 
      (PNET)-like component (GBM-PNET). These subgroups differ in their CD133/CD15 
      expression pattern and resemble cells with different stem-like genotype and 
      developmental pathway activation levels. Strikingly, clonogenic analysis of 
      cultures differentially expressing the investigated markers enabled the 
      identification of distinct subpopulations of cells endowed with stem cell 
      characteristics. High clonogenicity could be found in CD133(-)/CD15(-) and 
      CD133(+)/CD15(+) but not in CD133(-)/CD15(+) cells. Moreover, cell subpopulations 
      with pronounced clonogenic growth were characterized by high expression of stem 
      cell-related genes. Interestingly, these observations were unique for GBM-PNET 
      and differed from ordinary GBM cultures derived from tumours lacking a PNET 
      component. This work elucidates the complex molecular heterogeneity of in vitro 
      propagated glioblastoma-derived cells and potentially contributes to the 
      development of novel diagnostic modalities aiming at the identification of the 
      brain tumour stem-like cell population in a subgroup of GBMs.
FAU - Kahlert, Ulf D
AU  - Kahlert UD
AD  - Division of Stereotactic Neurosurgery, Department of General Neurosurgery, 
      University Medical Center Freiburg, Freiburg, Germany.
FAU - Bender, Noemi O
AU  - Bender NO
FAU - Maciaczyk, Donata
AU  - Maciaczyk D
FAU - Bogiel, Tomasz
AU  - Bogiel T
FAU - Bar, Eli E
AU  - Bar EE
FAU - Eberhart, Charles G
AU  - Eberhart CG
FAU - Nikkhah, Guido
AU  - Nikkhah G
FAU - Maciaczyk, Jaroslaw
AU  - Maciaczyk J
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Folia Neuropathol
JT  - Folia neuropathologica
JID - 9437431
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Glycoproteins)
RN  - 0 (Lewis X Antigen)
RN  - 0 (PROM1 protein, human)
RN  - 0 (Peptides)
RN  - EC 2.4.1.- (FUT4 protein, human)
RN  - EC 2.4.1.- (Fucosyltransferases)
SB  - IM
MH  - AC133 Antigen
MH  - Aged
MH  - Antigens, CD/analysis/biosynthesis
MH  - Biomarkers, Tumor/*analysis
MH  - Brain Neoplasms/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Flow Cytometry
MH  - Fucosyltransferases/analysis/biosynthesis
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Glioblastoma/metabolism/*pathology
MH  - Glycoproteins/analysis/biosynthesis
MH  - Humans
MH  - Lewis X Antigen/analysis/biosynthesis
MH  - Male
MH  - Neoplastic Stem Cells/metabolism/*pathology
MH  - Neuroectodermal Tumors, Primitive/metabolism/*pathology
MH  - Peptides/analysis
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/metabolism
EDAT- 2013/01/16 06:00
MHDA- 2013/11/14 06:00
CRDT- 2013/01/16 06:00
PHST- 2013/01/16 06:00 [entrez]
PHST- 2013/01/16 06:00 [pubmed]
PHST- 2013/11/14 06:00 [medline]
AID - 19883 [pii]
AID - 10.5114/fn.2012.32365 [doi]
PST - ppublish
SO  - Folia Neuropathol. 2012;50(4):357-68. doi: 10.5114/fn.2012.32365.