PMID- 23320952
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130207
LR  - 20211021
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 6
IP  - 1
DP  - 2013 Jan 16
TI  - Trisomy 8: a common finding in mouse embryonic stem (ES) cell lines.
PG  - 3
LID - 10.1186/1755-8166-6-3 [doi]
AB  - BACKGROUND: Obtaining a germ cell line is one of the most important steps in 
      developing a transgenic or knockout mouse with a targeted mutated gene of 
      interest. A common problem with this technology is that embryonic stem (ES) cells 
      often lack, or are extremely inefficient at, germ line transmission. RESULTS: To 
      determine whether chromosomal anomalies are correlated with inefficient ES cell 
      germ line transmission, we examined 97 constructed ES cell lines using 
      conventional cytogenetic analysis, and fluorescence in situ hybridization (FISH). 
      Chromosomal abnormalities occurred in 44 (45%) out of the 97 specimens analyzed: 
      31 specimens had trisomy 8 or mosaic trisomy 8, eight specimens had partial 
      trisomy 8 resulting from unbalanced translocations, and five specimens had other 
      chromosomal anomalies. CONCLUSIONS: Our data suggest that chromosomal analysis is 
      an important tool for improving the yield and quality of gene targeting 
      experiments.
FAU - Kim, Young Mi
AU  - Kim YM
AD  - Department of Pediatrics, The University of Oklahoma Health Sciences Center, 
      Oklahoma City, OK 73104, USA. shibo-li@ouhsc.edu.
FAU - Lee, Ji-Yun
AU  - Lee JY
FAU - Xia, Lijun
AU  - Xia L
FAU - Mulvihill, John J
AU  - Mulvihill JJ
FAU - Li, Shibo
AU  - Li S
LA  - eng
PT  - Journal Article
DEP - 20130116
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC3564830
EDAT- 2013/01/17 06:00
MHDA- 2013/01/17 06:01
PMCR- 2013/01/16
CRDT- 2013/01/17 06:00
PHST- 2012/08/28 00:00 [received]
PHST- 2012/12/06 00:00 [accepted]
PHST- 2013/01/17 06:00 [entrez]
PHST- 2013/01/17 06:00 [pubmed]
PHST- 2013/01/17 06:01 [medline]
PHST- 2013/01/16 00:00 [pmc-release]
AID - 1755-8166-6-3 [pii]
AID - 10.1186/1755-8166-6-3 [doi]
PST - epublish
SO  - Mol Cytogenet. 2013 Jan 16;6(1):3. doi: 10.1186/1755-8166-6-3.