PMID- 23322690
OWN - NLM
STAT- MEDLINE
DCOM- 20130408
LR  - 20240210
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 43
IP  - 1
DP  - 2013 Jan
TI  - Advantages and limitations of mouse models to deplete dendritic cells.
PG  - 22-6
LID - 10.1002/eji.201243022 [doi]
AB  - Dendritic cells (DCs) play a key role in regulating innate and adaptive immunity. 
      Our understanding of DC biology has benefited from studies in CD11c.DTR and 
      CD11c.DOG mouse models that use the CD11c promoter to express a diphtheria toxin 
      (DT) receptor transgene to inducibly deplete CD11c(+) cells. Other models to 
      inducibly deplete specific DC subsets upon administration of DT have also been 
      generated. However, most models suffer from limitations such as depletion of 
      additional cell types or the requirement to be used as radiation chimeras. 
      Moreover, CD11c.DTR and CD11c.DOG mice have recently been reported to display 
      neutrophilia and monocytosis upon DT injection. We discuss here some of the 
      limitations that should be taken into consideration when interpreting results 
      obtained with mouse models of DC ablation.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - van Blijswijk, Janneke
AU  - van Blijswijk J
AD  - Immunobiology Laboratory, London Research Institute, Cancer Research UK, London, 
      United Kingdom.
FAU - Schraml, Barbara U
AU  - Schraml BU
FAU - Reis e Sousa, Caetano
AU  - Reis e Sousa C
LA  - eng
GR  - 15689/CRUK_/Cancer Research UK/United Kingdom
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (CD11c Antigen)
RN  - 0 (Diphtheria Toxin)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/genetics/*metabolism
MH  - Dendritic Cells/*immunology
MH  - Diphtheria Toxin/genetics/metabolism
MH  - Humans
MH  - *Immunity, Innate
MH  - *Mice
MH  - Mice, Transgenic
MH  - *Models, Animal
MH  - Monocytes/immunology
MH  - Neutrophils/immunology
MH  - Promoter Regions, Genetic/genetics
EDAT- 2013/01/17 06:00
MHDA- 2013/04/09 06:00
CRDT- 2013/01/17 06:00
PHST- 2012/09/25 00:00 [received]
PHST- 2012/11/14 00:00 [accepted]
PHST- 2013/01/17 06:00 [entrez]
PHST- 2013/01/17 06:00 [pubmed]
PHST- 2013/04/09 06:00 [medline]
AID - 10.1002/eji.201243022 [doi]
PST - ppublish
SO  - Eur J Immunol. 2013 Jan;43(1):22-6. doi: 10.1002/eji.201243022.