PMID- 23323327
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20161125
IS  - 0869-2092 (Print)
IS  - 0869-2092 (Linking)
VI  - 75
IP  - 11
DP  - 2012
TI  - [Comparative in vitro study of anticoagulant activity of RA36 DNA aptamer in 
      human, rabbit, and rat blood plasma].
PG  - 13-8
AB  - DNA aptamer RA36 with a molecular weight of 10000 is direct-acting anticoagulant 
      whose efficacy is lower than that of recombinant hirudin and unfractionated 
      heparin (UFH) in blood clotting time (BCT), activated blood recalcification time 
      (ABRT), recalcification time (RT), prothrombin time (PT), and activated partial 
      thromboplastin time (APTT) tests. The anticoagulant effect of RA36 is comparable 
      with that of UFH in the thrombin time (TT) test, but is lower than the effect of 
      recombinant hirudin. Analysis of the blood and plasma anticoagulant activity 
      during intravenous bolus administration of aptamer RA36 in rabbits and rats is 
      based on the use ABRT (in blood case) and APTT/RT (in plasma case) tests. The 
      range of doses for evaluation of pharmacodynamic parameters of RA36 during 
      intravenous bolus administration in rabbits and rats is 3 - 34 mg/kg and 1 - 27 
      mg/kg, respectively. Accordingly, designed dose range for humans is 1 -29 mg/kg.
FAU - Savchik, E Iu
AU  - Savchik EIu
FAU - Kalinina, T B
AU  - Kalinina TB
FAU - Drozd, N N
AU  - Drozd NN
FAU - Makarov, V A
AU  - Makarov VA
FAU - Zav'ialova, E G
AU  - Zav'ialova EG
FAU - Lapsheva, E N
AU  - Lapsheva EN
FAU - Babii, A V
AU  - Babii AV
FAU - Mudrik, N N
AU  - Mudrik NN
FAU - Pavlova, G V
AU  - Pavlova GV
FAU - Golovin, A V
AU  - Golovin AV
FAU - Kopylov, A M
AU  - Kopylov AM
LA  - rus
PT  - Comparative Study
PT  - Journal Article
PL  - Russia (Federation)
TA  - Eksp Klin Farmakol
JT  - Eksperimental'naia i klinicheskaia farmakologiia
JID - 9215981
RN  - 0 (Anticoagulants)
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Hirudins)
RN  - 0 (Recombinant Proteins)
RN  - 9005-49-6 (Heparin)
RN  - Y43GF64R34 (lepirudin)
SB  - IM
MH  - Animals
MH  - Anticoagulants/chemical synthesis/pharmacokinetics/*pharmacology
MH  - Aptamers, Nucleotide/chemical synthesis/pharmacokinetics/*pharmacology
MH  - Blood Coagulation/*drug effects
MH  - Drug Dosage Calculations
MH  - Heparin/pharmacokinetics/pharmacology
MH  - Hirudins/pharmacokinetics/pharmacology
MH  - Humans
MH  - Injections, Intravenous
MH  - Male
MH  - Molecular Weight
MH  - Partial Thromboplastin Time
MH  - Prothrombin Time
MH  - Rabbits
MH  - Rats
MH  - Recombinant Proteins/pharmacokinetics/pharmacology
EDAT- 2013/01/18 06:00
MHDA- 2013/01/25 06:00
CRDT- 2013/01/18 06:00
PHST- 2013/01/18 06:00 [entrez]
PHST- 2013/01/18 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
PST - ppublish
SO  - Eksp Klin Farmakol. 2012;75(11):13-8.