PMID- 23323745
OWN - NLM
STAT- MEDLINE
DCOM- 20130927
LR  - 20130305
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 54
IP  - 2
DP  - 2013
TI  - Vaspin attenuates RANKL-induced osteoclast formation in RAW264.7 cells.
PG  - 147-52
LID - 10.3109/03008207.2012.761978 [doi]
AB  - Visceral adipose tissue-derived serine protease inhibitor (vaspin), an adipokine 
      that was recently identified in a rat model of type 2 diabetes, has been 
      suggested to have an insulin-sensitizing effect. In this study, we investigated 
      whether vaspin inhibits receptor activator of nuclear factor-kappaB ligand 
      (RANKL)-induced osteoclastogenesis using two types of osteoclast precursors: 
      RAW264.7 cells and bone marrow cells (BMCs). Vaspin inhibited RANKL-induced 
      osteoclastogenesis in RAW264.7 cells and BMCs. Interestingly, vaspin also 
      inhibited the RANKL-induced expression of nuclear factor of activated T cells c1 
      (NFATc1) in RAW264.7 cells and BMCs. Furthermore, it inhibited the RANKL-induced 
      upregulation of matrix metalloproteinase-9 and cathepsin K in RAW264.7 cells. 
      Thus, we suggest that vaspin downregulates osteoclastogenesis in part by 
      inhibiting expression of the transcription factor NFATc1.
FAU - Kamio, Noriaki
AU  - Kamio N
AD  - Department of Microbiology, Nihon University School of Dentistry, Tokyo, Japan. 
      kamio.noriaki@nihon-u.ac.jp
FAU - Kawato, Takayuki
AU  - Kawato T
FAU - Tanabe, Natsuko
AU  - Tanabe N
FAU - Kitami, Satoshi
AU  - Kitami S
FAU - Morita, Toyoko
AU  - Morita T
FAU - Ochiai, Kuniyasu
AU  - Ochiai K
FAU - Maeno, Masao
AU  - Maeno M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Adipokines)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (RANK Ligand)
RN  - 0 (Serpins)
RN  - 0 (vaspin protein, mouse)
RN  - EC 3.4.22.38 (Cathepsin K)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adipokines/*metabolism
MH  - Animals
MH  - Bone Marrow Cells/drug effects/metabolism
MH  - Cathepsin K/metabolism
MH  - Cell Line
MH  - Humans
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Mice
MH  - NFATC Transcription Factors/metabolism
MH  - Osteoclasts/*cytology/drug effects/enzymology/*metabolism
MH  - RANK Ligand/*pharmacology
MH  - Rats
MH  - Serpins/*metabolism
EDAT- 2013/01/18 06:00
MHDA- 2013/09/28 06:00
CRDT- 2013/01/18 06:00
PHST- 2013/01/18 06:00 [entrez]
PHST- 2013/01/18 06:00 [pubmed]
PHST- 2013/09/28 06:00 [medline]
AID - 10.3109/03008207.2012.761978 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2013;54(2):147-52. doi: 10.3109/03008207.2012.761978.