PMID- 23324601
OWN - NLM
STAT- MEDLINE
DCOM- 20131129
LR  - 20211021
IS  - 1555-8584 (Electronic)
IS  - 1547-6286 (Print)
IS  - 1547-6286 (Linking)
VI  - 10
IP  - 2
DP  - 2013 Feb
TI  - Structural organization of the polysomes adjacent to mammalian processing bodies 
      (P-bodies).
PG  - 314-20
LID - 10.4161/rna.23342 [doi]
AB  - A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is 
      important for the regulation of gene expression. In eukaryotic cells, this takes 
      place in dynamic cytoplasmic RNA-protein granules termed Processing bodies 
      (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and 
      template matching, we analyze the size and the organization of the polysomes in 
      the vicinity of human P-bodies. Our results show the presence of several 
      polysomes that are compatible with a translational activity around P-bodies. 
      Therefore, movement of mRNAs between polysomes and P-bodies can take place when 
      the two compartments are in close contact. The presence of initiation factors in 
      the proximity of P-bodies also suggests that translation of mRNAs can resume at 
      the periphery of these granules.
FAU - Cougot, Nicolas
AU  - Cougot N
AD  - Universite de Rennes 1, UMR, CNRS 6290 IGDR, Translation and Folding Team, Campus 
      de Beaulieu, Rennes Cedex, France.
FAU - Molza, Anne-Elisabeth
AU  - Molza AE
FAU - Giudice, Emmanuel
AU  - Giudice E
FAU - Cavalier, Annie
AU  - Cavalier A
FAU - Thomas, Daniel
AU  - Thomas D
FAU - Gillet, Reynald
AU  - Gillet R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130116
PL  - United States
TA  - RNA Biol
JT  - RNA biology
JID - 101235328
RN  - 0 (EIF4G2 protein, human)
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Eukaryotic Initiation Factor-4G)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cytoplasmic Granules/genetics/*metabolism
MH  - Electron Microscope Tomography
MH  - Eukaryotic Initiation Factor-4E/genetics/metabolism
MH  - Eukaryotic Initiation Factor-4G/genetics/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Polyribosomes/genetics/*metabolism/ultrastructure
MH  - Protein Biosynthesis
MH  - RNA Stability
MH  - *RNA Transport
MH  - RNA, Messenger/genetics/metabolism
PMC - PMC3594289
OTO - NOTNLM
OT  - P-bodies
OT  - electron tomography
OT  - initiation
OT  - mRNA
OT  - polysomes
OT  - ribosomes
EDAT- 2013/01/18 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/02/01
CRDT- 2013/01/18 06:00
PHST- 2013/01/18 06:00 [entrez]
PHST- 2013/01/18 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - 23342 [pii]
AID - 2012RNABIOL0302R [pii]
AID - 10.4161/rna.23342 [doi]
PST - ppublish
SO  - RNA Biol. 2013 Feb;10(2):314-20. doi: 10.4161/rna.23342. Epub 2013 Jan 16.