PMID- 23325335
OWN - NLM
STAT- MEDLINE
DCOM- 20130718
LR  - 20220316
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Linking)
VI  - 140
IP  - 10-12
DP  - 2012 Dec
TI  - Repetitive sequences associated with differentiation of W chromosome in 
      Semaprochilodus taeniurus.
PG  - 505-12
LID - 10.1007/s10709-013-9699-4 [doi]
AB  - The possible origins and differentiation of a ZZ/ZW sex chromosome system in 
      Semaprochilodus taeniurus, the only species of the family Prochilodontidae known 
      to possess heteromorphic sex chromosomes, were examined by conventional 
      (C-banding) and molecular (cross-species hybridization of W-specific WCP, 
      Fluorescence in situ hybridization (FISH) with telomere (TTAGGG)n, and Rex1 
      probes) cytogenetic protocols. Several segments obtained by W-specific probe were 
      cloned, and the sequences localized on the W chromosome were identified by DNA 
      sequencing and search of nucleotide collections of the NCBI and GIRI using BLAST 
      and CENSOR, respectively. Blocks of constitutive heterochromatin in chromosomes 
      of S. taeniurus were observed in the centromere of all autosomal chromosomes and 
      in the terminal, interstitial, and pericentromeric regions of the W chromosome, 
      which did not demonstrate interstitial telomeric sites with FISH of the telomere 
      probe. The Rex1 probe displayed a compartmentalized distribution pattern in some 
      chromosomes and showed signs of invasion of the pericentromeric region in the W 
      chromosome. Chromosomal painting with the W-specific WCP of S. taeniurus onto its 
      own chromosomes showed complete staining of the W chromosome, centromeric sites, 
      and the ends of the Z chromosome, as well as other autosomes. However, 
      cross-species painting using this WCP on chromosomes of S. insignis, Prochilodus 
      lineatus, and P. nigricans did not reveal a proto-W element, but instead 
      demonstrated scattered positive signals of repetitive DNAs. Identification of the 
      W-specific repetitive sequences showed high similarity to microsatellites and 
      transposable elements. Classes of repetitive DNA identified in the W chromosome 
      suggested that the genetic degeneration of this chromosome in S. taeniurus 
      occurred through accumulation of these repetitive DNAs.
FAU - Terencio, Maria Leandra
AU  - Terencio ML
AD  - Laboratorio de Genetica Animal, Instituto Nacional de Pesquisas da Amazonia, Av. 
      Andre Araujo, 2936, Petropolis, Manaus, AM, 69011-970, Brazil. 
      leandra@inpa.gov.br
FAU - Schneider, Carlos Henrique
AU  - Schneider CH
FAU - Gross, Maria Claudia
AU  - Gross MC
FAU - Nogaroto, Viviane
AU  - Nogaroto V
FAU - de Almeida, Mara Cristina
AU  - de Almeida MC
FAU - Artoni, Roberto Ferreira
AU  - Artoni RF
FAU - Vicari, Marcelo Ricardo
AU  - Vicari MR
FAU - Feldberg, Eliana
AU  - Feldberg E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130117
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (Heterochromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Characiformes/*genetics
MH  - Chromosome Painting/methods
MH  - Cloning, Molecular
MH  - DNA/genetics/*isolation & purification
MH  - Female
MH  - Genomics
MH  - Heterochromatin/genetics/isolation & purification
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Sex Chromosomes/*genetics
MH  - Telomere/genetics
EDAT- 2013/01/18 06:00
MHDA- 2013/07/19 06:00
CRDT- 2013/01/18 06:00
PHST- 2012/08/03 00:00 [received]
PHST- 2013/01/08 00:00 [accepted]
PHST- 2013/01/18 06:00 [entrez]
PHST- 2013/01/18 06:00 [pubmed]
PHST- 2013/07/19 06:00 [medline]
AID - 10.1007/s10709-013-9699-4 [doi]
PST - ppublish
SO  - Genetica. 2012 Dec;140(10-12):505-12. doi: 10.1007/s10709-013-9699-4. Epub 2013 
      Jan 17.