PMID- 23326191
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20211021
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 8
DP  - 2013
TI  - Increased healthy osteoblast to osteosarcoma density ratios on specific PLGA 
      nanopatterns.
PG  - 159-66
LID - 10.2147/IJN.S36408 [doi]
AB  - Poly(lactic-co-glycolic acid) ([PLGA] 50:50 wt% PLA:PGA) films with a flat 
      surface and with 27 nm, 190 nm, 300 nm, 400 nm, and 520 nm nanopatterns were 
      fabricated using a cast-mold process. The nanopatterns were transferred from 
      self-assembled polystyrene (PS) beads to PLGA films through polydimethylsiloxane 
      (PDMS) molds. The surface features, root-mean- square (RMS) roughness, and 
      wettability of these PLGA surface features were studied by atomic force 
      microscope (AFM) height scans, AFM z-sensor scans, and water contact angles, 
      respectively. In order to evaluate the influence of the material topography alone 
      (without changes in chemistry) for bone-cancer applications, both human healthy 
      osteoblasts and human cancerous osteosarcoma cells were cultured on these PLGA 
      surface features, and their densities were determined. Most importantly, compared 
      to all other substrates, it was found that the 27 nm PLGA nanopatterns 
      significantly increased the healthy osteoblast-to-osteosarcoma cell-density 
      ratio. For these reasons, and since previous studies have highlighted that 
      similar nanometer PLGA surface features decreased functions of other types of 
      cancerous cells (specifically lung and breast), this study suggests that 27 nm 
      PLGA nanopatterns should be further studied for a wide range of bone-cancer 
      applications, particularly where healthy bone-cell functions need to be promoted 
      over cancerous bone-cell functions.
FAU - Wang, Yongchen
AU  - Wang Y
AD  - Department of Chemistry, Brown University, Providence, RI, USA.
FAU - Zhang, Lijuan
AU  - Zhang L
FAU - Sun, Linlin
AU  - Sun L
FAU - Webster, Thomas J
AU  - Webster TJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130107
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Cell Count
MH  - Cell Culture Techniques/*instrumentation/methods
MH  - Cell Line, Tumor
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Microscopy, Atomic Force
MH  - Nanostructures/chemistry/*ultrastructure
MH  - Osteoblasts/*cytology
MH  - Osteosarcoma/*pathology
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Wettability
PMC - PMC3544334
OTO - NOTNLM
OT  - anti-cancer
OT  - bone
OT  - cancer
OT  - polymers
OT  - surface nanometer features
OT  - surface wettability
EDAT- 2013/01/18 06:00
MHDA- 2013/09/17 06:00
PMCR- 2013/01/07
CRDT- 2013/01/18 06:00
PHST- 2013/01/18 06:00 [entrez]
PHST- 2013/01/18 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
PHST- 2013/01/07 00:00 [pmc-release]
AID - ijn-8-159 [pii]
AID - 10.2147/IJN.S36408 [doi]
PST - ppublish
SO  - Int J Nanomedicine. 2013;8:159-66. doi: 10.2147/IJN.S36408. Epub 2013 Jan 7.