PMID- 23326811
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130118
LR  - 20231106
IS  - 2277-9175 (Print)
IS  - 2277-9175 (Electronic)
IS  - 2277-9175 (Linking)
VI  - 1
DP  - 2012
TI  - Determination and comparison of specifics of nucleus pulposus cells of human 
      intervertebral disc in alginate and chitosan-gelatin scaffolds.
PG  - 81
LID - 10.4103/2277-9175.102996 [doi]
LID - 81
AB  - INTRODUCTION: Low back pain is a major economical and social problem nowadays. 
      Intervertebral disc herniation and central degeneration of disc are two major 
      reasons of low back pain that occur because of structural impairment of disc. The 
      intervertebral disc contains three parts as follows : Annulus fibrosus, 
      transitional region, and nucleus pulposus, which forms the central nucleus of the 
      disc. The reduction of cell count and extracellular matrix, especially in nucleus 
      pulposus, causes disc degeneration. Different scaffolds (natural and synthetic) 
      have been used for tissue repairing and regeneration of the intervertebral disc 
      in tissue engineering. Most scaffolds have biodegradable and biocompatible 
      characteristics and also prepare a fine condition for proliferation and migration 
      of cells. In this study, proliferation of NP cells of human intervertebral disc 
      compromised in Chitosan-gelatin scaffold with alginate scaffold was studied. 
      MATERIALS AND METHODS: NP cells derived from nucleus pulposus by collagenase 
      enzymatic hydrolysis. They were derived from patients who undergoing open surgery 
      for discectomy in the Isfahan Alzahra hospital. Chitosan was blended with gelatin 
      and glutaraldehyde was used for cross linking the two polymers. Then, alginate 
      scaffold was prepared. Cellular suspension with 1 x 10(5) transferred to each 
      scaffold and cultured for 21 days. Cell viability and proliferation investigated 
      by trypan blue and (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 
      (MTT) assay. Scanning electron microscope (SEM) was used to assert the porosity 
      and to survey structure of scaffold. RESULTS: MTT assay dem1onstrated that cell 
      viability of third day had significant difference in contrast by first day in 
      both scaffolds. Accordingly, there was a significant decreased in cellular 
      viability from day 3 to 21. Results of the cell count showed a punctual elevation 
      cell numbers for alginate scaffold but there was no similar result for 
      chitosan-gelatin scaffold. CONCLUSION: Alginate scaffold prepared a better 
      condition for proliferation of NP cells in comparison with chitosan-gelatin 
      scaffold. Results of this study suggest that alginate scaffold could be useful in 
      in vivo studies and treatment.
FAU - Renani, Hamid Bahramian
AU  - Renani HB
AD  - Department of Anatomy and Molecular Biology, School of Medicine, Isfahan, Iran.
FAU - Ghorbani, Masood
AU  - Ghorbani M
FAU - Beni, Batool Hashemibeni
AU  - Beni BH
FAU - Karimi, Z
AU  - Karimi Z
FAU - Mirhosseini, Mm
AU  - Mirhosseini M
FAU - Zarkesh, H
AU  - Zarkesh H
FAU - Kabiri, A
AU  - Kabiri A
LA  - eng
PT  - Journal Article
DEP - 20121031
PL  - India
TA  - Adv Biomed Res
JT  - Advanced biomedical research
JID - 101586897
PMC - PMC3544085
OTO - NOTNLM
OT  - Alginate scaffold
OT  - NP cells
OT  - chitosan-gelatin scaffold
OT  - intervertebral disc
OT  - tissue enginiaring
COIS- Conflict of Interest: None declared
EDAT- 2013/01/18 06:00
MHDA- 2013/01/18 06:01
PMCR- 2012/10/31
CRDT- 2013/01/18 06:00
PHST- 2011/12/20 00:00 [received]
PHST- 2012/02/23 00:00 [accepted]
PHST- 2013/01/18 06:00 [entrez]
PHST- 2013/01/18 06:00 [pubmed]
PHST- 2013/01/18 06:01 [medline]
PHST- 2012/10/31 00:00 [pmc-release]
AID - ABR-1-81 [pii]
AID - 10.4103/2277-9175.102996 [doi]
PST - ppublish
SO  - Adv Biomed Res. 2012;1:81. doi: 10.4103/2277-9175.102996. Epub 2012 Oct 31.