PMID- 23327606
OWN - NLM
STAT- MEDLINE
DCOM- 20130711
LR  - 20211021
IS  - 1465-993X (Electronic)
IS  - 1465-9921 (Print)
IS  - 1465-9921 (Linking)
VI  - 14
IP  - 1
DP  - 2013 Jan 18
TI  - Human leukocyte antigen-G expression in differentiated human airway epithelial 
      cells: lack of modulation by Th2-associated cytokines.
PG  - 4
LID - 10.1186/1465-9921-14-4 [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA)-G is a nonclassical class I antigen 
      with immunomodulatory roles including up-regulation of suppressor T regulatory 
      lymphocytes. HLA-G was recently identified as an asthma susceptibility gene, and 
      expression of a soluble isoform, HLA-G5, has been demonstrated in human airway 
      epithelium. Increased presence of HLA-G5 has been demonstrated in bronchoalveolar 
      lavage fluid recovered from patients with mild asthma; this suggests a role for 
      this isoform in modulating airway inflammation though the mechanisms by which 
      this occurs is unclear. Airway inflammation associated with Th2 cytokines such as 
      IL-4 and IL-13 is a principal feature of asthma, but whether these cytokines 
      elicit expression of HLA-G is not known. METHODS: We examined gene and protein 
      expression of both soluble (G5) and membrane-bound (G1) HLA-G isoforms in primary 
      differentiated human airway epithelial cells collected from normal lungs and 
      grown in air-liquid interface culture. Cells were treated with up to 10 ng/ml of 
      either IL-4, IL-5, or IL-13, or 100 ng/ml of the immunomodulatory cytokine IL-10, 
      or 10,000 U/ml of the Th1-associated cytokine interferon-beta, for 24 hr, after 
      which RNA was isolated for evaluation by quantitative PCR and protein was 
      collected for Western blot analysis. RESULTS: HLA-G5 but not G1 was present in 
      dAEC as demonstrated by quantitative PCR, western blot and confocal microscopy. 
      Neither G5 nor G1 expression was increased by the Th2-associated cytokines IL-4, 
      IL-5 or IL-13 over 24 hr, nor after treatment with IL-10, but was increased 4.5 +/- 
      1.4 fold after treatment with 10,000 U/ml interferon-beta. CONCLUSIONS: These 
      data demonstrate the constitutive expression of a T lymphocyte regulatory 
      molecule in differentiated human airway epithelial cells that is not modulated by 
      Th2-associated cytokines.
FAU - White, Steven R
AU  - White SR
AD  - University of Chicago, Section of Pulmonary and Critical Care Medicine, Chicago, 
      IL 60637, USA. swhite@medicine.bsd.uchicago.edu
FAU - Loisel, Dagan A
AU  - Loisel DA
FAU - Stern, Randi
AU  - Stern R
FAU - Laxman, Bharathi
AU  - Laxman B
FAU - Floreth, Timothy
AU  - Floreth T
FAU - Marroquin, Bertha A
AU  - Marroquin BA
LA  - eng
GR  - HL-095268/HL/NHLBI NIH HHS/United States
GR  - HL-072414/HL/NHLBI NIH HHS/United States
GR  - HL-080417/HL/NHLBI NIH HHS/United States
GR  - U19 AI095230/AI/NIAID NIH HHS/United States
GR  - AI-056352/AI/NIAID NIH HHS/United States
GR  - AI-095320/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130118
PL  - England
TA  - Respir Res
JT  - Respiratory research
JID - 101090633
RN  - 0 (Cytokines)
RN  - 0 (HLA-G Antigens)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/*immunology
MH  - Epithelial Cells/*cytology/*immunology
MH  - HLA-G Antigens/*immunology
MH  - Humans
MH  - Immunomodulation/*immunology
MH  - Respiratory Mucosa/cytology/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC3560103
EDAT- 2013/01/19 06:00
MHDA- 2013/07/13 06:00
PMCR- 2013/01/18
CRDT- 2013/01/19 06:00
PHST- 2012/10/03 00:00 [received]
PHST- 2013/01/11 00:00 [accepted]
PHST- 2013/01/19 06:00 [entrez]
PHST- 2013/01/19 06:00 [pubmed]
PHST- 2013/07/13 06:00 [medline]
PHST- 2013/01/18 00:00 [pmc-release]
AID - 1465-9921-14-4 [pii]
AID - 10.1186/1465-9921-14-4 [doi]
PST - epublish
SO  - Respir Res. 2013 Jan 18;14(1):4. doi: 10.1186/1465-9921-14-4.