PMID- 23327610
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20220410
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 14
IP  - 2
DP  - 2013 Feb 11
TI  - Enhanced antitumor activity of the photosensitizer meso-Tetra(N-methyl-4-pyridyl) 
      porphine tetra tosylate through encapsulation in antibody-targeted 
      chitosan/alginate nanoparticles.
PG  - 302-10
LID - 10.1021/bm301858a [doi]
AB  - meso-Tetra(N-methyl-4-pyridyl) porphine tetra tosylate (TMP) is a photosensitizer 
      that can be used in photodynamic therapy (PDT) to induce cell death through 
      generation of reactive oxygen species in targeted tumor cells. However, TMP is 
      highly hydrophilic, and therefore, its ability to accumulate intracellularly is 
      limited. In this study, a strategy to improve TMP uptake into cells has been 
      investigated by encapsulating the compound in a hydrogel-based chitosan/alginate 
      nanoparticle formulation. Nanoparticles of 560 nm in diameter entrapping 9.1 mug 
      of TMP per mg of formulation were produced and examined in cell-based assays. 
      These particles were endocytosed into human colorectal carcinoma HCT116 cells and 
      elicited a more potent photocytotoxic effect than free drug. Antibodies targeting 
      death receptor 5 (DR5), a cell surface apoptosis-inducing receptor up-regulated 
      in various types of cancer and found on HCT116 cells, were then conjugated onto 
      the particles. The conjugated antibodies further enhanced uptake and cytotoxic 
      potency of the nanoparticle. Taken together, these results show that 
      antibody-conjugated chitosan/alginate nanoparticles significantly enhanced the 
      therapeutic effectiveness of entrapped TMP. This novel approach provides a 
      strategy for providing targeted site-specific delivery of TMP and other 
      photosensitizer drugs to treat colorectal tumors using PDT.
FAU - Abdelghany, Sharif M
AU  - Abdelghany SM
AD  - School of Pharmacy, Centre for Cancer Research and Cell Biology, Queen's 
      University Belfast , 97 Lisburn Road, Belfast, United Kingdom.
FAU - Schmid, Daniela
AU  - Schmid D
FAU - Deacon, Jill
AU  - Deacon J
FAU - Jaworski, Jakub
AU  - Jaworski J
FAU - Fay, Francois
AU  - Fay F
FAU - McLaughlin, Kirsty M
AU  - McLaughlin KM
FAU - Gormley, Julie A
AU  - Gormley JA
FAU - Burrows, James F
AU  - Burrows JF
FAU - Longley, Daniel B
AU  - Longley DB
FAU - Donnelly, Ryan F
AU  - Donnelly RF
FAU - Scott, Christopher J
AU  - Scott CJ
LA  - eng
GR  - G1001805/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130131
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Alginates)
RN  - 0 (Antibodies)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Porphyrins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 38673-65-3 (tetra(4-N-methylpyridyl)porphine)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.4.22.- (Caspase 8)
SB  - IM
MH  - Alginates
MH  - Antibodies/immunology
MH  - Antineoplastic Agents/*therapeutic use
MH  - Biological Transport
MH  - Caspase 8/metabolism
MH  - Cell Line, Tumor
MH  - Chitosan/immunology
MH  - Colorectal Neoplasms/*drug therapy
MH  - Glucuronic Acid/immunology
MH  - Hexuronic Acids/immunology
MH  - Humans
MH  - Nanoparticles
MH  - Photochemotherapy
MH  - Photosensitizing Agents/*therapeutic use
MH  - Porphyrins/*therapeutic use
MH  - RNA Interference
MH  - RNA, Small Interfering
MH  - Reactive Oxygen Species/metabolism
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics/immunology
PMC - PMC3582313
EDAT- 2013/01/19 06:00
MHDA- 2013/08/02 06:00
CRDT- 2013/01/19 06:00
PHST- 2013/01/19 06:00 [entrez]
PHST- 2013/01/19 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
AID - 10.1021/bm301858a [doi]
PST - ppublish
SO  - Biomacromolecules. 2013 Feb 11;14(2):302-10. doi: 10.1021/bm301858a. Epub 2013 
      Jan 31.