PMID- 23327700
OWN - NLM
STAT- MEDLINE
DCOM- 20131114
LR  - 20130118
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 92
IP  - 48
DP  - 2012 Dec 25
TI  - [Clinicopathological significance of the expression of carbonic anhydrase I and 
      II in human pancreatic cancer].
PG  - 3407-11
AB  - OBJECTIVE: To explore the clinicopathological significance of the expression of 
      carbonic anhydrase I (CAI) and carbonic anhydrase II (CAII) protein and mRNA 
      levels in pancreatic ductal adenocarcinoma (PDAC). METHODS: The expression of CAI 
      and CAII protein in 57 pairs of paraffin-embedded PDAC specimens and adjacent 
      non-cancerous pancreatic tissues were detected by immunohistochemistry. The 
      relationship between the protein expression and clinicopathological characters 
      was analyzed. Western blot and quantitative real-time polymerase chain reaction 
      (QRT-PCR) were used to examine the expression of CAI and CAII protein and mRNA 
      levels in 16 paired fresh PDAC specimens, adjacent non-cancerous pancreatic 
      tissues and 3 different differentiated pancreatic cancer cells (PANC-1, BxPC-3 & 
      SW1990). RESULTS: The expression of CAI protein was higher in PDAC compared with 
      that in paired non-cancerous pancreatic tissues (t = 2.395, P = 0.020), whereas, 
      CAII expression was significantly lower in PDAC than that in paired non-cancerous 
      pancreatic tissues (t = 4.296, P = 0.000). The expression of CAI and CAII protein 
      had a positive correlation with tumor differentiation (chi(2) = 7.557, P = 0.023; 
      chi(2) = 7.822, P = 0.020) and CAI showed a direct negative correlation with 
      vascular invasion (chi(2) = 6.349, P = 0.012). Univariate and multivariate analysis 
      with clinicopathological characters revealed that the CAII expression was an 
      independent prognostic indicator for PDAC patients (P = 0.017; P = 0.011 
      respectively). No significant difference of CAI mRNA and protein expression 
      existed between PDAC and adjacent normal pancreatic tissues (t = 1.619, P = 
      0.126; t = 1.352, P = 0.197) as detected by Western blot and QRT-PCR, but the 
      expression of CAII mRNA and protein levels was much lower in PDAC than that in 
      paired non-cancerous pancreatic tissues, respectively (t = 3.360, P = 0.004; t = 
      2.934, P = 0.010). Moreover, the mRNA and protein expression of CAI and CAII 
      gradually increased with the better differentiation degree of pancreatic cancer 
      cells. CONCLUSIONS: CAI is up-regulated and CAII down-regulated in PDAC. Both of 
      them are correlated with tumor differentiation. CAII expression is an independent 
      prognostic indicator for PDAC patients.
FAU - Sheng, Wei-wei
AU  - Sheng WW
AD  - Department of General Surgery, First Hospital, China Medical University, 
      Shenyang, China.
FAU - Dong, Ming
AU  - Dong M
FAU - Zhou, Jian-ping
AU  - Zhou JP
FAU - Kong, Fan-min
AU  - Kong FM
FAU - Li, Yu-ji
AU  - Li YJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - EC 4.2.1.- (Carbonic Anhydrase I)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Aged
MH  - Carbonic Anhydrase I/*metabolism
MH  - Carbonic Anhydrase II/*metabolism
MH  - Carcinoma, Pancreatic Ductal/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Neoplasms/metabolism/*pathology
MH  - Prognosis
EDAT- 2013/01/19 06:00
MHDA- 2013/11/15 06:00
CRDT- 2013/01/19 06:00
PHST- 2013/01/19 06:00 [entrez]
PHST- 2013/01/19 06:00 [pubmed]
PHST- 2013/11/15 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2012 Dec 25;92(48):3407-11.