PMID- 23328256
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20131121
IS  - 1423-0216 (Electronic)
IS  - 1021-7401 (Linking)
VI  - 20
IP  - 2
DP  - 2013
TI  - Spinal cord brain-derived neurotrophic factor levels increase after dexamethasone 
      treatment in male rats with chronic inflammation.
PG  - 119-25
LID - 10.1159/000345995 [doi]
AB  - Dexamethasone is widely used in the therapy of chronic inflammatory diseases for 
      its pain-modulating effects. The objective of this study was to evaluate the 
      effect of dexamethasone on nociception and local inflammation, and the levels of 
      brain-derived neurotrophic factor (BDNF) in the spinal cord in male rats with 
      chronic inflammation induced by complete Freund's adjuvant (CFA). Rats were 
      randomly divided into a control group (not manipulated) and 2 CFA-induced chronic 
      inflammation groups (in the 15th post-CFA injection): 1 injected with vehicle 
      (saline solution) and 1 received dexamethasone (0.25 mg/kg) for 8 days. The 
      hot-plate and electronic von Frey tests were performed 24 h after the end of 
      treatment. BDNF spinal cord levels were determined by enzyme-linked immunosorbent 
      assay (ELISA). The level of inflammation in the tibiotarsal joint (the ankle 
      region) was evaluated histologically at the end of treatment. Dexamethasone 
      produced significantly increased latency in the hot-plate test (one-way ANOVA, p 
      < 0.05) and withdrawal threshold in the electronic von Frey test (p < 0.005). The 
      dexamethasone group showed increased spinal cord BDNF levels compared to the 
      other groups (one-way ANOVA p, < 0.05). Histological analysis showed a local 
      inflammatory response only in animals treated with vehicle, which demonstrated 
      that the dexamethasone treatment decreased the inflammatory process. Our findings 
      corroborate the antinociceptive and anti-inflammatory properties of 
      dexamethasone. In addition, we showed that the dexamethasone treatment increased 
      BDNF levels in the spinal cord; its pain- modulating effects can be attributed to 
      this effect.
CI  - Copyright (c) 2013 S. Karger AG, Basel.
FAU - Laste, Gabriela
AU  - Laste G
AD  - Laboratorio de Farmacologia da Dor, Departamento de Farmacologia, Instituto de 
      Ciencias Basicas da Saude, Porto Alegre, Brazil.
FAU - Ripoll Rozisky, Joanna
AU  - Ripoll Rozisky J
FAU - de Macedo, Isabel Cristina
AU  - de Macedo IC
FAU - Souza Dos Santos, Vinicius
AU  - Souza Dos Santos V
FAU - Custodio de Souza, Izabel Cristina
AU  - Custodio de Souza IC
FAU - Caumo, Wolnei
AU  - Caumo W
FAU - Torres, Iraci L S
AU  - Torres IL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130115
PL  - Switzerland
TA  - Neuroimmunomodulation
JT  - Neuroimmunomodulation
JID - 9422763
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/*cerebrospinal fluid
MH  - Chronic Disease
MH  - Dexamethasone/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Freund's Adjuvant/toxicity
MH  - Inflammation/cerebrospinal fluid/chemically induced/drug therapy
MH  - Male
MH  - Pain/*cerebrospinal fluid
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord/drug effects/metabolism
EDAT- 2013/01/19 06:00
MHDA- 2013/08/10 06:00
CRDT- 2013/01/19 06:00
PHST- 2012/09/11 00:00 [received]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2013/01/19 06:00 [entrez]
PHST- 2013/01/19 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 000345995 [pii]
AID - 10.1159/000345995 [doi]
PST - ppublish
SO  - Neuroimmunomodulation. 2013;20(2):119-25. doi: 10.1159/000345995. Epub 2013 Jan 
      15.