PMID- 2332835
OWN - NLM
STAT- MEDLINE
DCOM- 19900607
LR  - 20131121
IS  - 0031-9341 (Print)
IS  - 0031-9341 (Linking)
VI  - 52
IP  - 2
DP  - 1990
TI  - [Effect of intracellular organic phosphates on erythrocyte deformability].
PG  - 36-46
AB  - Organic phosphates in human erythrocytes were selectively varied by incubating 
      fresh human erythrocytes in phosphate-buffered saline containing inosine, 
      pyruvate, adenine, and/or adenosine in various concentrations. The deformability 
      of erythrocytes was measured at 24 degrees C with a rheoscope under shear stress 
      of 8-82 dyn/cm2. (1) With increasing 2, 3-DPG (5 approximately 15 mM/l cells), 
      undeformable erythrocytes increased due to the increased mean corpuscular 
      hemoglobin concentration (MCHC). However, these cells became deformable, when the 
      MCHC was reduced by suspending in hypotonic medium. (2) At the same MCHC, the 
      deformability of 2, 3-DPG-enriched erythrocytes was still reduced, compared with 
      that of control erythrocytes, probably due to altered membrane viscoelastic 
      properties. (3) 2, 3-DPG-reduced erythrocytes (2.2 mM/l cells) was not altered in 
      their deformability. (4) Deformability of 2, 3-DPG-enriched erythrocytes was not 
      changed by lowering oxygen tension. (5) Deformability of erythrocytes was not 
      affected by varying intracellular ATP in the range of 0.5 approximately 2.2 mM/l 
      cells (ATP in control cells was 1 mM/l cells). (6) Increment of IMP 
      (approximately 0.9 mM/l cells) and ITP (approximately 0.5 mM/l cells) did not 
      alter the deformability of erythrocytes. (7) Interaction of intracellular organic 
      phosphates with membrane proteins was discussed.
FAU - Suzuki, Y
AU  - Suzuki Y
AD  - 2nd Department of Physiology, School of Medicine, Ehime University, Japan.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Nihon Seirigaku Zasshi
JT  - Nihon seirigaku zasshi. Journal of the Physiological Society of Japan
JID - 0417460
RN  - 0 (Diphosphoglyceric Acids)
RN  - 0 (Organophosphorus Compounds)
RN  - 131-99-7 (Inosine Monophosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adenosine Triphosphate/blood
MH  - Cells, Cultured
MH  - Diphosphoglyceric Acids/blood
MH  - *Erythrocyte Deformability
MH  - Erythrocyte Indices
MH  - Erythrocytes/*metabolism
MH  - Humans
MH  - Inosine Monophosphate/blood
MH  - Organophosphorus Compounds/*blood
MH  - Oxygen/blood
MH  - Rheology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Seirigaku Zasshi. 1990;52(2):36-46.