PMID- 23329458
OWN - NLM
STAT- MEDLINE
DCOM- 20130626
LR  - 20130118
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 949
DP  - 2013
TI  - Integrated Fluidic Circuits (IFCs) for digital PCR.
PG  - 423-31
LID - 10.1007/978-1-62703-134-9_27 [doi]
AB  - The Fluidigm Digital Array IFC is a nanofluidic biochip where digital PCR 
      reactions can be performed with isolated individual DNA template molecules. This 
      chip is part of a family of integrated fluidic circuits (IFC) and contains a 
      network of fluid lines, NanoFlex valves and chambers. NanoFlex valves are made 
      of an elastomeric material that deflects under pressure to create a tight seal 
      and are used to regulate the flow of liquids in the IFC. Digital Arrays have 
      enabled a different approach to digital PCR, by partitioning DNA molecules 
      instead of diluting them. Single DNA molecules are randomly distributed into 
      nanoliter volume reaction chambers and then PCR amplified in the presence of a 
      fluorophore-containing probe. Positive fluorescent signal indicates the presence 
      of a DNA molecule in a reaction chamber, while negative chambers are blank. IFC 
      technology enables the delivery of very precise volumes of solutions in a simple, 
      fast procedure, utilizing a minimum of sample and assay reagents. The development 
      of the IFC technology and the Digital Array chip has revolutionized the field of 
      biology, and has been utilized in gene copy number studies, absolute quantitation 
      (molecule counting) of genomic DNA and cDNA, rare mutation detection, and digital 
      haplotyping.
FAU - Ramakrishnan, Ramesh
AU  - Ramakrishnan R
AD  - Fluidigm Corporation, South San Francisco, CA, USA. 
      ramesh.ramakrishnan@fluidigm.com
FAU - Qin, Jian
AU  - Qin J
FAU - Jones, Robert C
AU  - Jones RC
FAU - Weaver, L Suzanne
AU  - Weaver LS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/genetics
MH  - Gene Dosage/genetics
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Nanotechnology/*instrumentation
MH  - Real-Time Polymerase Chain Reaction/*instrumentation
EDAT- 2013/01/19 06:00
MHDA- 2013/06/28 06:00
CRDT- 2013/01/19 06:00
PHST- 2013/01/19 06:00 [entrez]
PHST- 2013/01/19 06:00 [pubmed]
PHST- 2013/06/28 06:00 [medline]
AID - 10.1007/978-1-62703-134-9_27 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;949:423-31. doi: 10.1007/978-1-62703-134-9_27.