PMID- 23329502
OWN - NLM
STAT- MEDLINE
DCOM- 20130625
LR  - 20170823
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 960
DP  - 2013
TI  - Preparing antigens suitable for cross-presentation assays in vitro and in vivo.
PG  - 389-400
LID - 10.1007/978-1-62703-218-6_29 [doi]
AB  - Cross-presentation is defined as the ability of certain professional 
      antigen-presenting cells to take up, process and present extracellular antigens 
      on major histocompatibility class I (MHC-I) molecules to CD8+ T cells. The 
      stimulation of naive cytotoxic CD8+ T cells by this process, termed 
      cross-priming, is involved in many different responses, including those to 
      tumors, pathogens, graft tissues, and self-antigens. Dendritic cells (DCs), a 
      heterogeneous cell population, are endowed with the highest cross-priming 
      capacity. Investigation of their cross-presentation capacities, important both 
      for vaccination and for the induction of immune tolerance can be performed by in 
      vivo and in vitro assays. In this chapter we describe the preparation of antigens 
      that can be used to test cross-presentation via pinocytosis, receptor-mediated 
      endocytosis, and phagocytosis.
FAU - Saveanu, Loredana
AU  - Saveanu L
AD  - Faculte de medecine Rene Descartes, INSERM U1013 and Universite Paris Descartes, 
      Sorbonne Paris Cite, Paris, France. loredana.saveanu@inserm.fr.
FAU - van Endert, Peter
AU  - van Endert P
AD  - Faculte de medecine Rene Descartes, INSERM U1013 and Universite Paris Descartes, 
      Sorbonne Paris Cite, Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Latex)
RN  - 0 (Receptors, Fc)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - *Cross-Priming
MH  - Dendritic Cells/cytology/immunology/metabolism
MH  - Latex/chemistry
MH  - Mice
MH  - Microspheres
MH  - Ovalbumin/chemistry/*immunology/*metabolism
MH  - Phagocytosis
MH  - Pinocytosis
MH  - Receptors, Fc/metabolism
MH  - Solubility
MH  - Yeasts/cytology
EDAT- 2013/01/19 06:00
MHDA- 2013/06/26 06:00
CRDT- 2013/01/19 06:00
PHST- 2013/01/19 06:00 [entrez]
PHST- 2013/01/19 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - 10.1007/978-1-62703-218-6_29 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;960:389-400. doi: 10.1007/978-1-62703-218-6_29.