PMID- 23332974
OWN - NLM
STAT- MEDLINE
DCOM- 20130628
LR  - 20131121
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 99
IP  - 5
DP  - 2013 Apr
TI  - Different levels of leptin regulate different target enzymes involved in 
      progesterone synthesis.
PG  - 1460-6
LID - S0015-0282(12)02523-X [pii]
LID - 10.1016/j.fertnstert.2012.12.014 [doi]
AB  - OBJECTIVE: To study the effects of different doses of leptin on the expression of 
      proteins involved in P synthesis, such as steroidogenic acute regulatory protein 
      (StAR), cytochrome P450 side chain cleavage (P450scc), and 3beta-hydroxysteroid 
      dehydrogenase (3betaHSD). DESIGN: Experimental studies. SETTING: Research 
      laboratory. ANIMAL(S): Immature rats primed with gonadotropins to induce 
      ovulation. INTERVENTION(S): In vivo studies: rats received either an acute or 
      daily treatment with leptin. In vitro studies: ovarian explants were cultured in 
      the absence or presence of leptin (0.3-500 ng/mL). MAIN OUTCOME MEASURE(S): The 
      expression of both messenger RNA and protein of StAR, P450scc, and 3betaHSD were 
      measured by reverse transcription-polymerase chain reaction (PCR) and Western 
      blot, respectively. RESULT(S): The acute treatment with leptin, which inhibits 
      the ovulatory process, caused a significant reduction in the ovarian expression 
      of P450scc without changes in StAR or 3betaHSD. In contrast, the daily treatment, 
      which induces the ovulatory process, showed an increased expression of the 
      ovarian 3betaHSD protein, without differences in the other proteins measured. We 
      also found that leptin increased the protein of both P450scc and 3betaHSD at 
      physiological levels and inhibited both messenger RNA and protein of 3betaHSD at 
      higher concentrations. CONCLUSION(S): The results indicate that 1) leptin is able 
      to regulate the expression of the 3betaHSD protein in a dose-dependent manner; and 
      2) leptin seems to exert its dual effects on P synthesis on different targets in 
      a dose-dependent manner.
CI  - Copyright (c) 2013 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Bilbao, Maria Guillermina
AU  - Bilbao MG
AD  - Centro de Estudios Farmacologicos y Botanicos (CEFYBO-CONICET), Facultad de 
      Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Di Yorio, Maria Paula
AU  - Di Yorio MP
FAU - Faletti, Alicia Graciela
AU  - Faletti AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130116
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Gonadotropins)
RN  - 0 (Leptin)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*genetics/metabolism
MH  - Age Factors
MH  - Animals
MH  - Cholesterol Side-Chain Cleavage Enzyme/*genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gene Expression Regulation, Enzymologic/drug effects/physiology
MH  - Gonadotropins/pharmacology
MH  - Leptin/pharmacology/*physiology
MH  - Ovulation/drug effects/physiology
MH  - Ovulation Induction/methods
MH  - Phosphoproteins/*genetics/metabolism
MH  - Progesterone/*biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2013/01/22 06:00
MHDA- 2013/07/03 06:00
CRDT- 2013/01/22 06:00
PHST- 2012/08/27 00:00 [received]
PHST- 2012/11/29 00:00 [revised]
PHST- 2012/12/10 00:00 [accepted]
PHST- 2013/01/22 06:00 [entrez]
PHST- 2013/01/22 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - S0015-0282(12)02523-X [pii]
AID - 10.1016/j.fertnstert.2012.12.014 [doi]
PST - ppublish
SO  - Fertil Steril. 2013 Apr;99(5):1460-6. doi: 10.1016/j.fertnstert.2012.12.014. Epub 
      2013 Jan 16.