PMID- 23333087
OWN - NLM
STAT- MEDLINE
DCOM- 20140129
LR  - 20211203
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 24
IP  - 7
DP  - 2013 Jul
TI  - Maternal postnatal high-fat diet, rather than gestational diet, affects 
      morphology and mTOR pathway in skeletal muscle of weaning rat.
PG  - 1340-8
LID - S0955-2863(12)00286-0 [pii]
LID - 10.1016/j.jnutbio.2012.10.009 [doi]
AB  - The positive regulation of insulin pathway in skeletal muscle results in 
      increased activity of the mammalian target of rapamycin (mTOR), a positive 
      effector of mRNA translation rate and protein synthesis. Studies that assess the 
      activity of this protein in response to chronic high-fat diet (HFD) are scarce 
      and controversial, and to date, there are no studies evaluating the mTOR pathway 
      in infants exposed to gestational and postgestational HFD. This study 
      investigated the effect of maternal HFD on skeletal muscle morphology and on 
      phosphorylation of proteins that comprise the intracellular mTOR signaling 
      pathway in soleus muscle of offspring at weaning. For this purpose, 10 days prior 
      to conception, 39 female Wistar rats were randomly assigned to either control 
      diet (CTL) or HFD. Later, rats were distributed into four groups according to 
      gestational and postpregnancy diet: CTL/CTL (n=10), CTL/HF (n=11), HF/HF (n=10) 
      and HF/CTL (n=8). After 21 days of lactation, pups were killed, and blood samples 
      and soleus and gastrocnemius skeletal muscle were collected for analysis. We 
      observed an influence of maternal postgestational diet, rather than gestational 
      diet, in promoting an obese phenotype, characterized by body fat accumulation, 
      insulin resistance and high serum leptin, glucose, triglycerides and cholesterol 
      levels (P<.05). We have also detected alterations on skeletal muscle 
      morphology--with reduced myofiber density--and impairment on S6 kinase 1 and 4E 
      binding protein-1 phosphorylation (P<.05). These results emphasize the importance 
      of maternal diet during lactation on muscle morphology and on physiological 
      adaptations of infant rats.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Pantaleao, Lucas C
AU  - Pantaleao LC
AD  - Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical 
      Sciences, University of Sao Paulo, Sao Paulo, Brazil. lucascp@usp.br
FAU - Teodoro, Gabriela F R
AU  - Teodoro GF
FAU - Torres-Leal, Francisco L
AU  - Torres-Leal FL
FAU - Vianna, Daiana
AU  - Vianna D
FAU - de Paula, Tatyana D
AU  - de Paula TD
FAU - de Matos-Neto, Emidio M
AU  - de Matos-Neto EM
FAU - Trindade, Michele C C
AU  - Trindade MC
FAU - Rogero, Marcelo M
AU  - Rogero MM
FAU - Bueno, Carlos R Jr
AU  - Bueno CR Jr
FAU - Tirapegui, Julio
AU  - Tirapegui J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130117
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Dietary Fats)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Body Composition
MH  - Dietary Fats/*administration & dosage
MH  - Female
MH  - Insulin Resistance
MH  - Litter Size
MH  - Muscle, Skeletal/anatomy & histology/*metabolism
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - *Weaning
EDAT- 2013/01/22 06:00
MHDA- 2014/01/30 06:00
CRDT- 2013/01/22 06:00
PHST- 2012/04/01 00:00 [received]
PHST- 2012/10/18 00:00 [revised]
PHST- 2012/10/29 00:00 [accepted]
PHST- 2013/01/22 06:00 [entrez]
PHST- 2013/01/22 06:00 [pubmed]
PHST- 2014/01/30 06:00 [medline]
AID - S0955-2863(12)00286-0 [pii]
AID - 10.1016/j.jnutbio.2012.10.009 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2013 Jul;24(7):1340-8. doi: 10.1016/j.jnutbio.2012.10.009. Epub 
      2013 Jan 17.