PMID- 23335099
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR  - 20171116
IS  - 1098-2396 (Electronic)
IS  - 0887-4476 (Linking)
VI  - 67
IP  - 5
DP  - 2013 May
TI  - Effects of antipsychotic drugs on the expression of synaptic proteins and 
      dendritic outgrowth in hippocampal neuronal cultures.
PG  - 224-34
LID - 10.1002/syn.21634 [doi]
AB  - Recent evidence has suggested that atypical antipsychotic drugs regulate synaptic 
      plasticity. We investigated whether some atypical antipsychotic drugs 
      (olanzapine, aripiprazole, quetiapine, and ziprasidone) altered the expression of 
      synapse-associated proteins in rat hippocampal neuronal cultures under toxic 
      conditions induced by B27 deprivation. A typical antipsychotic, haloperidol, was 
      used for comparison. We measured changes in the expression of various synaptic 
      proteins including postsynaptic density protein-95 (PSD-95), brain-derived 
      neurotrophic factor (BDNF), and synaptophysin (SYP). Then we examined whether 
      these drugs affected the dendritic morphology of hippocampal neurons. We found 
      that olanzapine, aripiprazole, and quetiapine, but not haloperidol, significantly 
      hindered the B27 deprivation-induced decrease in the levels of these synaptic 
      proteins. Ziprasidone did not affect PSD-95 or BDNF levels, but significantly 
      increased the levels of SYP under B27 deprivation conditions. Moreover, 
      olanzapine and aripiprazole individually significantly increased the levels of 
      PSD-95 and BDNF, respectively, even under normal conditions, whereas haloperidol 
      decreased the levels of PSD-95. These drugs increased the total outgrowth of 
      hippocampal dendrites via PI3K signaling, whereas haloperidol had no effect in 
      this regard. Together, these results suggest that the up-regulation of synaptic 
      proteins and dendritic outgrowth may represent key effects of some atypical 
      antipsychotic drugs but that haloperidol may be associated with distinct actions.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Park, Sung Woo
AU  - Park SW
AD  - Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
FAU - Lee, Chan Hong
AU  - Lee CH
FAU - Cho, Hye Yeon
AU  - Cho HY
FAU - Seo, Mi Kyoung
AU  - Seo MK
FAU - Lee, Jung Goo
AU  - Lee JG
FAU - Lee, Bong Ju
AU  - Lee BJ
FAU - Seol, Wongi
AU  - Seol W
FAU - Kee, Baik Seok
AU  - Kee BS
FAU - Kim, Young Hoon
AU  - Kim YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130214
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (Antipsychotic Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Synaptophysin)
SB  - IM
MH  - Animals
MH  - Antipsychotic Agents/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cells, Cultured
MH  - Dendrites/*drug effects/metabolism
MH  - Disks Large Homolog 4 Protein
MH  - Gene Expression/*drug effects
MH  - Hippocampus/*cytology/metabolism
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Membrane Proteins/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synaptophysin/genetics/*metabolism
MH  - Up-Regulation
EDAT- 2013/01/22 06:00
MHDA- 2013/09/04 06:00
CRDT- 2013/01/22 06:00
PHST- 2012/10/17 00:00 [received]
PHST- 2012/12/18 00:00 [accepted]
PHST- 2013/01/22 06:00 [entrez]
PHST- 2013/01/22 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
AID - 10.1002/syn.21634 [doi]
PST - ppublish
SO  - Synapse. 2013 May;67(5):224-34. doi: 10.1002/syn.21634. Epub 2013 Feb 14.