PMID- 23335531
OWN - NLM
STAT- MEDLINE
DCOM- 20130424
LR  - 20161125
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 17
IP  - 4
DP  - 2012 Oct-Dec
TI  - PTEN expression in non small cell lung carcinoma based on digitized image 
      analysis.
PG  - 719-23
AB  - PURPOSE: HER2 depended signalling pathway is dereg-ulated in a subset of non 
      small cell lung carcinoma (NSCLC). The tumor suppressor gene PTEN (10q21) 
      regulates the HER2/PI3K/Akt signalling pathway. Our aim was to evaluate PTEN 
      protein expression in NSCLC based on a quantitative analysis method correlating 
      also the results with clinicopathological parameters. METHODS: Protein expression 
      was determined by immunohistochemistry (IHC) in 61 paraffin-embedded cases of 
      patients with NSCLC. Digital image analysis (staining intensity levels) was 
      performed in the corresponding immunostained slides. RESULTS: Loss of PTEN 
      expression was observed in 24 (39.34%) cases, low expression in 29 (47.54%) and 
      overexpression in 8 (13.12%) cases. Multivariate analysis determined that PTEN 
      overexpression was associated with lower risk to develop metastases (p=0.05). 
      CONCLUSION: PTEN deregulation is a relatively frequent genetic event in NSCLC, 
      associated with progressive metastatic process in those patients. Because of 
      binding to the ErbB2 receptor, trastuzumab stabilizes and activates PTEN gene, 
      and loss of its expression negatively affects the response rates in such 
      patients.
FAU - Panagiotou, I
AU  - Panagiotou I
AD  - Department of Thoracic Surgery, 401 GA Hospital of Athens, Athens, Greece.
FAU - Tsiambas, E
AU  - Tsiambas E
FAU - Lazaris, A C
AU  - Lazaris AC
FAU - Kavantzas, N
AU  - Kavantzas N
FAU - Konstantinou, M
AU  - Konstantinou M
FAU - Kalkandi, P
AU  - Kalkandi P
FAU - Ragkos, V
AU  - Ragkos V
FAU - Metaxas, G E
AU  - Metaxas GE
FAU - Roukas, D K
AU  - Roukas DK
FAU - Vilaras, G
AU  - Vilaras G
FAU - Patsouris, E
AU  - Patsouris E
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*chemistry/pathology
MH  - Female
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Lung Neoplasms/*chemistry/pathology
MH  - Male
MH  - Middle Aged
MH  - PTEN Phosphohydrolase/*analysis
EDAT- 2013/01/22 06:00
MHDA- 2013/04/25 06:00
CRDT- 2013/01/22 06:00
PHST- 2013/01/22 06:00 [entrez]
PHST- 2013/01/22 06:00 [pubmed]
PHST- 2013/04/25 06:00 [medline]
PST - ppublish
SO  - J BUON. 2012 Oct-Dec;17(4):719-23.