PMID- 23335771
OWN - NLM
STAT- MEDLINE
DCOM- 20130806
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 79
IP  - 6
DP  - 2013 Mar
TI  - Characterization of the denitrification-associated phosphorus uptake properties 
      of "Candidatus Accumulibacter phosphatis" clades in sludge subjected to enhanced 
      biological phosphorus removal.
PG  - 1969-79
LID - 10.1128/AEM.03464-12 [doi]
AB  - To characterize the denitrifying phosphorus (P) uptake properties of "Candidatus 
      Accumulibacter phosphatis," a sequencing batch reactor (SBR) was operated with 
      acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to 
      anaerobic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate 
      concentration and the anoxic time. The communities of "Ca. Accumulibacter" and 
      associated bacteria at the initial (AO) and final (A2O) stages were compared 
      using 16S rRNA and polyphosphate kinase genes and using fluorescence in situ 
      hybridization (FISH). The acclimation process led to a clear shift in the 
      relative abundances of recognized "Ca. Accumulibacter" subpopulations from clades 
      IIA > IA > IIF to clades IIC > IA > IIF, as well as to increases in the abundance 
      of other associated bacteria (Dechloromonas [from 1.2% to 19.2%] and "Candidatus 
      Competibacter phosphatis" [from 16.4% to 20.0%]), while the overall "Ca. 
      Accumulibacter" abundance decreased (from 55.1% to 29.2%). A series of batch 
      experiments combined with FISH/microautoradiography (MAR) analyses was performed 
      to characterize the denitrifying P uptake properties of the "Ca. Accumulibacter" 
      clades. In FISH/MAR experiments using slightly diluted sludge ( approximately 0.5 g/liter), all 
      "Ca. Accumulibacter" clades successfully took up phosphorus in the presence of 
      nitrate. However, the "Ca. Accumulibacter" clades showed no P uptake in the 
      presence of nitrate when the sludge was highly diluted ( approximately 0.005 g/liter); under 
      these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake 
      by "Ca. Accumulibacter" clades occurred when nitrite was added. These results 
      suggest that the "Ca. Accumulibacter" cells lack nitrate reduction capabilities 
      and that P uptake by "Ca. Accumulibacter" is dependent upon nitrite generated by 
      associated nitrate-reducing bacteria such as Dechloromonas and "Ca. 
      Competibacter."
FAU - Kim, Jeong Myeong
AU  - Kim JM
AD  - Department of Life Science & Research Center for Biomolecules and Biosystems, 
      Chung-Ang University, Seoul, Republic of Korea.
FAU - Lee, Hyo Jung
AU  - Lee HJ
FAU - Lee, Dae Sung
AU  - Lee DS
FAU - Jeon, Che Ok
AU  - Jeon CO
LA  - eng
SI  - GENBANK/JQ726360
SI  - GENBANK/JQ726361
SI  - GENBANK/JQ726362
SI  - GENBANK/JQ726363
SI  - GENBANK/JQ726364
SI  - GENBANK/JQ726365
SI  - GENBANK/JQ726366
SI  - GENBANK/JQ726367
SI  - GENBANK/JQ726368
SI  - GENBANK/JQ726369
SI  - GENBANK/JQ726370
SI  - GENBANK/JQ726371
SI  - GENBANK/JQ726372
SI  - GENBANK/JQ726373
SI  - GENBANK/JQ726374
SI  - GENBANK/JQ726375
SI  - GENBANK/JQ726376
SI  - GENBANK/JQ726377
SI  - GENBANK/JQ726378
SI  - GENBANK/JQ726379
SI  - GENBANK/JQ726380
SI  - GENBANK/JQ726381
SI  - GENBANK/JQ726382
SI  - GENBANK/JQ726383
SI  - GENBANK/JQ726384
SI  - GENBANK/JQ726385
SI  - GENBANK/JQ726386
SI  - GENBANK/JQ726387
SI  - GENBANK/JQ726388
SI  - GENBANK/JQ726389
SI  - GENBANK/JQ726390
SI  - GENBANK/JQ726391
SI  - GENBANK/JQ726392
SI  - GENBANK/JQ726393
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130118
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Acetates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Acetates/metabolism
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Betaproteobacteria/isolation & purification/*metabolism
MH  - Bioreactors/microbiology
MH  - Biota
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - *Denitrification
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phosphorus/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
PMC - PMC3592233
EDAT- 2013/01/22 06:00
MHDA- 2013/08/07 06:00
PMCR- 2013/09/01
CRDT- 2013/01/22 06:00
PHST- 2013/01/22 06:00 [entrez]
PHST- 2013/01/22 06:00 [pubmed]
PHST- 2013/08/07 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - AEM.03464-12 [pii]
AID - 03464-12 [pii]
AID - 10.1128/AEM.03464-12 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2013 Mar;79(6):1969-79. doi: 10.1128/AEM.03464-12. Epub 
      2013 Jan 18.