PMID- 23336943 OWN - NLM STAT- MEDLINE DCOM- 20130617 LR - 20130204 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1278 DP - 2013 Feb 22 TI - Analysis of preservatives with different polarities in beverage samples by dual-phase dual stir bar sorptive extraction combined with high-performance liquid chromatography. PG - 8-15 LID - S0021-9673(13)00025-3 [pii] LID - 10.1016/j.chroma.2012.12.061 [doi] AB - A new concept of "dual-phase dual stir bar sorptive extraction (SBSE)" was proposed to simultaneously extract six preservatives with different polarities (logKo/w values of 1.27-3.41), namely, benzoic acid (BA), sorbic acid (SA), methyl p-hydroxybenzoate (MP), ethyl p-hydroxybenzoate (EP), propyl p-hydroxybenzoate (PP), and butyl p-hydroxybenzoate (BP). The dual-phase dual SBSE apparatus was consisted of two differently coated stir bars, a 3-aminopropyltriethoxysilane (APTES)-hydroxy-terminated silicone oil (OH-TSO)-coated stir bar that was prepared by sol-gel technique and a C(18) silica (C(18))-polydimethylsiloxane (PDMS)-coated stir bar that was prepared by adhesion. In dual-phase dual SBSE, the two stir bars with different coatings were placed in the same sample solution for the simultaneous extraction of the target analytes with different polarities, and then the bars were desorbed in the same desorption solvent. The extraction performance of the dual-phase dual SBSE for the six preservatives was evaluated by comparing with the conventional SBSE (individual stir bar) with different coatings, including commercial PDMS, homemade PDMS, C(18)-APTES-OH-TSO, APTES-OH-TSO, and C(18)-PDMS. The experimental results showed that the dual-phase dual SBSE had the highest extraction efficiency for the six target preservatives. Based on this fact, a novel method by combining the dual-phase dual SBSE which was consisted of the APTES-OH-TSO-coated and C(18)-PDMS-coated stir bars with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was developed for the simultaneous analysis of six target beverage preservatives in beverages. Under optimal conditions, the limits of detection (LODs) for six target preservatives ranged from 0.6 to 2.7 mugL(-1) with the relative standard deviations (RSDs) of 4.6-9.2% (C(BA,SA)=5 mugL(-1),C(MP)=20 mugL(-1),C(EP,PP,BP)=10 mugL(-1), n=7). The enrichment factors (EFs) were approximately 16-42-fold (theoretical EF was 50-fold). The proposed method was validated by the analysis of six target preservatives in three kinds of beverage samples, and the recoveries for the spiked samples were in the range of 76.6-118.6% for cola, 74.6-17.5% for orange juice, and 83.0-119.1% for herbal tea, respectively. CI - Copyright (c) 2013 Elsevier B.V. All rights reserved. FAU - Xu, Jin AU - Xu J AD - Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, China. FAU - Chen, Beibei AU - Chen B FAU - He, Man AU - He M FAU - Hu, Bin AU - Hu B LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130108 PL - Netherlands TA - J Chromatogr A JT - Journal of chromatography. A JID - 9318488 SB - IM MH - Beverages/*analysis MH - Chromatography, High Pressure Liquid/*methods MH - Hydrogen-Ion Concentration MH - Microscopy, Electron, Scanning MH - Osmolar Concentration MH - Spectrophotometry, Ultraviolet EDAT- 2013/01/23 06:00 MHDA- 2013/06/19 06:00 CRDT- 2013/01/23 06:00 PHST- 2012/09/13 00:00 [received] PHST- 2012/12/19 00:00 [revised] PHST- 2012/12/25 00:00 [accepted] PHST- 2013/01/23 06:00 [entrez] PHST- 2013/01/23 06:00 [pubmed] PHST- 2013/06/19 06:00 [medline] AID - S0021-9673(13)00025-3 [pii] AID - 10.1016/j.chroma.2012.12.061 [doi] PST - ppublish SO - J Chromatogr A. 2013 Feb 22;1278:8-15. doi: 10.1016/j.chroma.2012.12.061. Epub 2013 Jan 8.