PMID- 23337936
OWN - NLM
STAT- MEDLINE
DCOM- 20130812
LR  - 20190109
IS  - 1532-8651 (Electronic)
IS  - 1098-7339 (Linking)
VI  - 38
IP  - 2
DP  - 2013 Mar-Apr
TI  - Effects of chronic administration of amitriptyline, gabapentin and minocycline on 
      spinal brain-derived neurotrophic factor expression and neuropathic pain behavior 
      in a rat chronic constriction injury model.
PG  - 124-30
LID - 10.1097/AAP.0b013e31827d611b [doi]
AB  - BACKGROUND: In animal models of neuropathic pain (NP), promising results have 
      been reported with the administration of minocycline, possibly through inhibition 
      of spinal brain-derived neurotrophic factor (BDNF) expression. No data are 
      available on the effect of amitriptyline and gabapentin on spinal BDNF 
      expression. If the mechanism of action of the latter drugs does not involve 
      brain-derived NP inhibition, further clinical research in BDNF is warranted. 
      METHODS: In this placebo-controlled study, we investigated the effects of 
      amitriptyline (5 mg/kg), gabapentin (50 mg/kg), and minocycline (25 mg/kg) twice 
      a day on NP behavior in a sciatic chronic constriction injury (CCI) rat model. 
      Drug treatment started 7 days after CCI and lasted 14 days. At postoperative day 
      21, spinal BDNF expression in laminae I and II was quantified using 
      immunocytochemistry. RESULTS: Sciatic CCI resulted in NP behavior throughout the 
      duration of the experiment in the placebo group. When administered for 2 weeks, 
      minocycline (P </= 0.001) and amitriptyline (P </= 0.05), but not gabapentin, reduced 
      thermal hyperalgesia. None of these drugs reduced mechanical allodynia. As 
      opposed to amitriptyline and gabapentin, 2 weeks of treatment with minocycline 
      reduced brain-derived, neurotrophic factor immunoreactivity (P </= 0.05) in the 
      ipsilateral dorsal horn. CONCLUSIONS: Minocycline and amitriptyline both reduce 
      NP behavior in a sciatic CCI rat model, but only minocycline reduces spinal BDNF, 
      indicating different modes of action of these 2 drugs. The observed actions of 
      minocycline closely fit the clinical needs for the treatment of NP.
FAU - Vanelderen, Pascal
AU  - Vanelderen P
AD  - Department of Anesthesiology, Intensive Care, Emergency Care and Pain Therapy, 
      Ziekenhuis Oost-Limburg, Genk, Belgium. pascal.vanelderen@gmail.com
FAU - Rouwette, Tom
AU  - Rouwette T
FAU - Kozicz, Tamas
AU  - Kozicz T
FAU - Heylen, Rene
AU  - Heylen R
FAU - Van Zundert, Jan
AU  - Van Zundert J
FAU - Roubos, Eric W
AU  - Roubos EW
FAU - Vissers, Kris
AU  - Vissers K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Reg Anesth Pain Med
JT  - Regional anesthesia and pain medicine
JID - 9804508
RN  - 0 (Amines)
RN  - 0 (Analgesics)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclohexanecarboxylic Acids)
RN  - 1806D8D52K (Amitriptyline)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 6CW7F3G59X (Gabapentin)
RN  - FYY3R43WGO (Minocycline)
SB  - IM
MH  - Amines/*administration & dosage
MH  - Amitriptyline/*administration & dosage
MH  - Analgesics/*administration & dosage
MH  - Animals
MH  - Behavior, Animal/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Constriction
MH  - Cyclohexanecarboxylic Acids/*administration & dosage
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Drug Administration Schedule
MH  - Gabapentin
MH  - Hyperalgesia/diagnosis/*drug therapy/metabolism/physiopathology/psychology
MH  - Immunohistochemistry
MH  - Male
MH  - Minocycline/*administration & dosage
MH  - Neuralgia/diagnosis/*drug therapy/metabolism/physiopathology/psychology
MH  - Pain Measurement
MH  - Pain Perception/drug effects
MH  - Pain Threshold/drug effects
MH  - Posterior Horn Cells/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/surgery
MH  - Spinal Cord/*drug effects/metabolism/physiopathology
MH  - Time Factors
MH  - gamma-Aminobutyric Acid/*administration & dosage
EDAT- 2013/01/23 06:00
MHDA- 2013/08/13 06:00
CRDT- 2013/01/23 06:00
PHST- 2013/01/23 06:00 [entrez]
PHST- 2013/01/23 06:00 [pubmed]
PHST- 2013/08/13 06:00 [medline]
AID - 10.1097/AAP.0b013e31827d611b [doi]
PST - ppublish
SO  - Reg Anesth Pain Med. 2013 Mar-Apr;38(2):124-30. doi: 
      10.1097/AAP.0b013e31827d611b.