PMID- 23338489
OWN - NLM
STAT- MEDLINE
DCOM- 20130716
LR  - 20130129
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 42
IP  - 3
DP  - 2013 Mar
TI  - Potent growth-inhibitory effect of a dual cancer-specific oncolytic adenovirus 
      expressing apoptin on prostate carcinoma.
PG  - 1052-60
LID - 10.3892/ijo.2013.1783 [doi]
AB  - Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive 
      apoptotic protein with the ability to specifically induce apoptosis in various 
      human tumor cells, but not in normal cells. To explore the use of apoptin in 
      tumor gene therapy, we assessed a recombinant adenovirus expressing the apoptin 
      protein (Ad-hTERTp-E1a-Apoptin) in order to determine its lethal and 
      growth-inhibitory effects on PC-3 and RM-1 cells in vitro and its antitumor 
      effect on solid tumors in vivo. 
      3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), acridine 
      orange (AO)/ethidium bromide (EB), 4'-6-diamidino-2-phenylindole (DAPI), and 
      Annexin V assays showed that Ad-hTERTp-E1a-Apoptin inhibited the proliferation of 
      PC-3 and RM-1 cells in vitro by inducing apoptosis of prostate cancer cells, and 
      that this inhibitory effect was dose and time-dependent. In the animal models, 
      Ad-hTERTp-E1a-Apoptin significantly inhibited tumor growth and extended the 
      lifespan of animals. Experimental results indicate that Ad-hTERTp-E1a-Apoptin has 
      a potential application in tumor gene therapy.
FAU - Zhang, Muchun
AU  - Zhang M
AD  - Department of Urology, China-Japan Union Hospital of Jilin University, Changchun 
      130031, P.R. China.
FAU - Wang, Jinhui
AU  - Wang J
FAU - Li, Chang
AU  - Li C
FAU - Hu, Ningning
AU  - Hu N
FAU - Wang, Kai
AU  - Wang K
FAU - Ji, Huifan
AU  - Ji H
FAU - He, Dongyun
AU  - He D
FAU - Quan, Chengshi
AU  - Quan C
FAU - Li, Xiao
AU  - Li X
FAU - Jin, Ningyi
AU  - Jin N
FAU - Li, Yulin
AU  - Li Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130121
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Capsid Proteins)
RN  - 0 (VP3 protein, Chicken anemia virus)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - Capsid Proteins/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Chicken anemia virus/genetics/metabolism
MH  - Genetic Therapy/*methods
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - Oncolytic Viruses/genetics
MH  - Promoter Regions, Genetic
MH  - Prostatic Neoplasms/*therapy
MH  - Random Allocation
EDAT- 2013/01/23 06:00
MHDA- 2013/07/17 06:00
CRDT- 2013/01/23 06:00
PHST- 2012/11/01 00:00 [received]
PHST- 2012/12/17 00:00 [accepted]
PHST- 2013/01/23 06:00 [entrez]
PHST- 2013/01/23 06:00 [pubmed]
PHST- 2013/07/17 06:00 [medline]
AID - 10.3892/ijo.2013.1783 [doi]
PST - ppublish
SO  - Int J Oncol. 2013 Mar;42(3):1052-60. doi: 10.3892/ijo.2013.1783. Epub 2013 Jan 
      21.