PMID- 23339028
OWN - NLM
STAT- MEDLINE
DCOM- 20140414
LR  - 20181202
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 27
IP  - 10
DP  - 2013 Oct
TI  - Inhibiting roles of berberine in gut movement of rodents are related to 
      activation of the endogenous opioid system.
PG  - 1564-71
LID - 10.1002/ptr.4926 [doi]
AB  - Although Berberine (BER) is popular in treating gastrointestinal (GI) disorders, 
      its mechanisms are not clear yet. In order to investigate the effects and 
      possible mechanism of BER on GI motility in rodents, we first explored GI 
      motility by recording the myoelectrical activity of jejunum and colon in rats, 
      and upper GI transit with a charcoal marker in mice. Then, the plasma levels of 
      gastrin, motilin, somatostatin and glucagon-like-peptide-1 (Glp-1) were measured 
      by ELISA or radioimmunoassay (RIA). Furthermore, endogenous opioid-peptides 
      (beta-endorphin, dynorphin-A, met-enkephalin) were detected by RIA after treatment 
      with BER. Our results showed that BER concentration-dependently inhibited 
      myoelectrical activity and GI transit, which can be antagonized by 
      opioid-receptor antagonists to different extents. The elevated somatostatin and 
      Glp-1, and decreased gastrin and motilin in plasma, which were caused by BER 
      application, also could be antagonized by the opioid-receptor antagonists. 
      Additionally, plasma level of beta-endorphin, but not dynorphin-A and 
      met-enkephalin, was increased by applying BER. Taken together, these studies show 
      that BER plays inhibiting roles on GI motility and up-regulating roles on 
      somatostatin, Glp-1 and down-regulating roles on gastrin, motilin. The 
      pharmacological mechanisms of BER on GI motility and plasma levels of GI hormones 
      were discovered to be closely related to endogenous opioid system.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Feng, Yajing
AU  - Feng Y
AD  - Department of Pathophysiology, Institute of Digestive Disease, Tongji University 
      School of Medicine, Shanghai, 200092, China.
FAU - Li, Yongyu
AU  - Li Y
FAU - Chen, Chunqiu
AU  - Chen C
FAU - Lin, Xuhong
AU  - Lin X
FAU - Yang, Yuehua
AU  - Yang Y
FAU - Cai, Haidong
AU  - Cai H
FAU - Lv, Zhongwei
AU  - Lv Z
FAU - Cao, Minghua
AU  - Cao M
FAU - Li, Kun
AU  - Li K
FAU - Xu, Jing
AU  - Xu J
FAU - Li, Sainan
AU  - Li S
FAU - Jia, Yijun
AU  - Jia Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130121
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Gastrins)
RN  - 0 (Gastrointestinal Hormones)
RN  - 0 (Opioid Peptides)
RN  - 0I8Y3P32UF (Berberine)
RN  - 51110-01-1 (Somatostatin)
RN  - 52906-92-0 (Motilin)
RN  - 58569-55-4 (Enkephalin, Methionine)
RN  - 60617-12-1 (beta-Endorphin)
RN  - 74913-18-1 (Dynorphins)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
SB  - IM
MH  - Animals
MH  - Berberine/*pharmacology
MH  - Colon/drug effects/physiology
MH  - Dynorphins/physiology
MH  - Enkephalin, Methionine/physiology
MH  - Gastrins/physiology
MH  - Gastrointestinal Hormones/*physiology
MH  - Gastrointestinal Motility/*drug effects
MH  - Gastrointestinal Tract/*drug effects/physiology
MH  - Gastrointestinal Transit/drug effects/physiology
MH  - Glucagon-Like Peptide 1/physiology
MH  - Jejunum/drug effects/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Motilin/physiology
MH  - Opioid Peptides/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Somatostatin/physiology
MH  - beta-Endorphin/physiology
OTO - NOTNLM
OT  - Berberine
OT  - endogenous opioid system
OT  - gastrointestinal hormones
OT  - gastrointestinal motility
EDAT- 2013/01/23 06:00
MHDA- 2014/04/15 06:00
CRDT- 2013/01/23 06:00
PHST- 2012/10/29 00:00 [received]
PHST- 2012/12/06 00:00 [revised]
PHST- 2012/12/19 00:00 [accepted]
PHST- 2013/01/23 06:00 [entrez]
PHST- 2013/01/23 06:00 [pubmed]
PHST- 2014/04/15 06:00 [medline]
AID - 10.1002/ptr.4926 [doi]
PST - ppublish
SO  - Phytother Res. 2013 Oct;27(10):1564-71. doi: 10.1002/ptr.4926. Epub 2013 Jan 21.