PMID- 23339137
OWN - NLM
STAT- MEDLINE
DCOM- 20141105
LR  - 20181202
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 29
IP  - 10
DP  - 2014 Oct
TI  - An "ex vivo" model to evaluate toxicological responses to mixtures of 
      contaminants in cetaceans: integumentum biopsy slices.
PG  - 1107-21
LID - 10.1002/tox.21841 [doi]
AB  - The need for powerful new tools to detect the effects of chemical pollution, in 
      particular of persistent organic pollutants (POPs) and polycyclic aromatic 
      hydrocarbons (PAHs) on Mediterranean cetaceans led us to develop and apply a 
      suite of sensitive biomarkers for integument biopsies of stranded and 
      free-ranging animals. This multi-response ex vivo method has the aim to detect 
      toxicological effects of contaminant mixtures. In the present study, we applied 
      an ex vivo assay using skin biopsy and liver slices, combining molecular 
      biomarkers [Western blot of Cytochrome P450 1A1 (CYP1A1) and Cytochrome P450 2B 
      (CYP2B)] and gene expression biomarkers (Quantitative real-time PCR of CYP1A1, 
      heat shock protein 70, estrogen receptor alpha and E2F transcription factor) in 
      response to chemical exposure [organochlorines compounds (OCs), polybrominated 
      diphenyl ethers (PBDEs), and PAHs] for stranded Mediterranean Stenella 
      coeruleoalba. The main goal of this experiment was to identify the biomarker 
      and/or a suite of biomarkers that could best detect the presence of a specific 
      class of pollutants (OCs, PBDEs, and PAHs) or a mixture of them. This 
      multi-response biomarker methodology revealed an high sensitivity and selectivity 
      of responses (such as CYP1A and ER alpha mRNA variations after OCs and PAHs exposure) 
      and could represent a valid future approach for the study of inter- and 
      intra-species sensitivities to various classes of environmental contaminants.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc., a Wiley company.
FAU - Fossi, Maria Cristina
AU  - Fossi MC
AD  - Department of Earth, Physical and Environmental Sciences, University of Siena, 
      Via P.A. Mattioli 4, 53100 Siena, Italy.
FAU - Casini, Silvia
AU  - Casini S
FAU - Maltese, Silvia
AU  - Maltese S
FAU - Panti, Cristina
AU  - Panti C
FAU - Spinsanti, Giacomo
AU  - Spinsanti G
FAU - Marsili, Letizia
AU  - Marsili L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130122
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/analysis/genetics
MH  - Biopsy
MH  - Estrogen Receptor alpha/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Halogenated Diphenyl Ethers/*toxicity
MH  - Hydrocarbons, Chlorinated/*toxicity
MH  - Liver/drug effects/metabolism
MH  - Polycyclic Aromatic Hydrocarbons/*toxicity
MH  - Real-Time Polymerase Chain Reaction
MH  - Skin/drug effects/metabolism
MH  - Stenella/genetics/*physiology
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Mediterranean cetaceans
OT  - Stenella coeruleoalba
OT  - biological response
OT  - lipophilic contaminant
OT  - skin organotypic cultures
OT  - treatments
EDAT- 2013/01/23 06:00
MHDA- 2014/11/06 06:00
CRDT- 2013/01/23 06:00
PHST- 2012/02/14 00:00 [received]
PHST- 2012/12/10 00:00 [revised]
PHST- 2012/12/10 00:00 [accepted]
PHST- 2013/01/23 06:00 [entrez]
PHST- 2013/01/23 06:00 [pubmed]
PHST- 2014/11/06 06:00 [medline]
AID - 10.1002/tox.21841 [doi]
PST - ppublish
SO  - Environ Toxicol. 2014 Oct;29(10):1107-21. doi: 10.1002/tox.21841. Epub 2013 Jan 
      22.