PMID- 23339567
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20220309
IS  - 1742-2094 (Electronic)
IS  - 1742-2094 (Linking)
VI  - 10
DP  - 2013 Jan 22
TI  - Monocyte chemoattractant protein-1 affects migration of hippocampal neural 
      progenitors following status epilepticus in rats.
PG  - 11
LID - 10.1186/1742-2094-10-11 [doi]
AB  - BACKGROUND: Epilepsy is a common brain disorder characterized by a chronic 
      predisposition to generate spontaneous seizures. The mechanisms for epilepsy 
      formation remain unknown. A growing body of evidence suggests the involvement of 
      inflammatory processes in epileptogenesis. In the present study, we investigated 
      the involvement of monocyte chemoattractant protein-1 (MCP-1) in aberrant 
      migration of hippocampal progenitors in rats after the insult of status 
      epilepticus (SE). METHODS: SE was induced with pilocarpine in Sprague-Dawley 
      rats. Transcriptional expression of MCP-1 in the dentate gyrus (DG) was measured 
      using quantitative real-time PCR. From 1 to 28 days after SE, the temporal 
      profiles of MCP-1 protein expression in DG were evaluated using enzyme-linked 
      immunosorbent assay. Chemokine (C-C motif) receptor 2 (CCR2) expression in 
      doublecortin-positive neuronal progenitors was examined using double-labeling 
      immunohistochemistry. The involvement of MCP-1/CCR2 signaling in aberrant 
      neuronal progenitor migration in the epileptic hippocampus was assessed in the SE 
      rats using a CCR2 antagonist, RS102895, and the ectopic migration of neuronal 
      progenitors was determined using Prox1/doublecortin double immunostaining. 
      RESULTS: After SE, MCP-1 gene was significantly upregulated and its corresponding 
      protein expression in the DG was significantly increased on days 1 and 3. Some 
      hilar ectopic progenitor cells of SE rats expressed the MCP-1 receptor, CCR2. 
      Notably, the ectopic migration of neuronal progenitors into hilus was attenuated 
      by a blockade of the MCP-1/CCR2 interaction with a selective CCR2 inhibitor, 
      RS102895. CONCLUSIONS: An increase in dentate MCP-1 is associated with 
      seizure-induced aberrant migration of neuronal progenitors through the 
      interaction with CCR2. The upregulation of MCP-1 after an insult of SE may play a 
      role in the generation of epilepsy.
FAU - Hung, Yu-Wen
AU  - Hung YW
AD  - Institute of Physiology, National Yang-Ming University, No,155, Sec, 2, Linong 
      Street, Taipei, 112, Taiwan.
FAU - Lai, Ming-Tsong
AU  - Lai MT
FAU - Tseng, Yi-Jhan
AU  - Tseng YJ
FAU - Chou, Chien-Chen
AU  - Chou CC
FAU - Lin, Yung-Yang
AU  - Lin YY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130122
PL  - England
TA  - J Neuroinflammation
JT  - Journal of neuroinflammation
JID - 101222974
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
SB  - IM
MH  - Animals
MH  - Cell Movement/*physiology
MH  - Chemokine CCL2/*biosynthesis
MH  - Doublecortin Protein
MH  - Hippocampus/*metabolism/pathology
MH  - Male
MH  - Neural Stem Cells/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Status Epilepticus/*metabolism/pathology
PMC - PMC3563591
EDAT- 2013/01/24 06:00
MHDA- 2013/10/23 06:00
PMCR- 2013/01/22
CRDT- 2013/01/24 06:00
PHST- 2012/01/19 00:00 [received]
PHST- 2013/01/07 00:00 [accepted]
PHST- 2013/01/24 06:00 [entrez]
PHST- 2013/01/24 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
PHST- 2013/01/22 00:00 [pmc-release]
AID - 1742-2094-10-11 [pii]
AID - 10.1186/1742-2094-10-11 [doi]
PST - epublish
SO  - J Neuroinflammation. 2013 Jan 22;10:11. doi: 10.1186/1742-2094-10-11.