PMID- 23341142
OWN - NLM
STAT- MEDLINE
DCOM- 20131204
LR  - 20231213
IS  - 1473-4230 (Electronic)
IS  - 1473-4222 (Linking)
VI  - 12
IP  - 3
DP  - 2013 Jun
TI  - Coupling between GABA-A receptor and chloride transporter underlies ionic 
      plasticity in cerebellar Purkinje neurons.
PG  - 328-30
LID - 10.1007/s12311-013-0453-3 [doi]
AB  - Ionic plasticity, a form of synaptic plasticity unique to inhibitory 
      neurotransmission, can be induced in cerebellar Purkinje neurons by brain-derived 
      neurotrophic factor (BDNF). It is expressed as a decrease in synaptic strength of 
      GABA-A transmission onto Purkinje neurons due to reduced transmembrane chloride 
      gradient. By making whole-cell recordings, we found that the effect of BDNF is 
      mediated by neuronal potassium and chloride transporter KCC2 because it is 
      blocked by inhibitors of KCC2 or by raising the intracellular chloride 
      concentration. Under these conditions in which KCC2 activity is reduced, BDNF 
      augments evoked GABA-A currents suggesting a direct facilitatory effect of BDNF 
      on GABA-A receptor. We also found that the effect of BDNF is highly localized at 
      the GABA-A synapse and is secured by physical coupling between GABA-A receptor 
      and KCC2, as revealed by coimmunoprecipitation studies. Based on these results, 
      we hypothesize that the interaction between KCC2 and specific subunit of GABA-A 
      receptor represents a fundamental mechanism rendering the rapid induction of 
      ionic plasticity in individual or input-specific GABA synapses possible. Such a 
      mechanism may be important for the function of Purkinje neurons that are known to 
      express GABA-A receptors with different subunit compositions.
FAU - Huang, Ying
AU  - Huang Y
AD  - Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan 
      University, Shanghai, China.
FAU - Wang, Jian-Jun
AU  - Wang JJ
FAU - Yung, Wing-Ho
AU  - Yung WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cerebellum
JT  - Cerebellum (London, England)
JID - 101089443
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Symporters)
SB  - IM
MH  - Animals
MH  - Cerebellum/*cytology
MH  - Immunoprecipitation
MH  - In Vitro Techniques
MH  - Models, Neurological
MH  - Neuronal Plasticity/*physiology
MH  - Patch-Clamp Techniques
MH  - Purkinje Cells/*physiology
MH  - Rats
MH  - Receptors, GABA-A/*metabolism
MH  - Symporters/*metabolism
MH  - K Cl- Cotransporters
EDAT- 2013/01/24 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/01/24 06:00
PHST- 2013/01/24 06:00 [entrez]
PHST- 2013/01/24 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1007/s12311-013-0453-3 [doi]
PST - ppublish
SO  - Cerebellum. 2013 Jun;12(3):328-30. doi: 10.1007/s12311-013-0453-3.