PMID- 23341914
OWN - NLM
STAT- MEDLINE
DCOM- 20130709
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 1
DP  - 2013
TI  - Transcriptional profiling of human dendritic cell populations and models--unique 
      profiles of in vitro dendritic cells and implications on functionality and 
      applicability.
PG  - e52875
LID - 10.1371/journal.pone.0052875 [doi]
LID - e52875
AB  - BACKGROUND: Dendritic cells (DCs) comprise heterogeneous populations of cells, 
      which act as central orchestrators of the immune response. Applicability of 
      primary DCs is restricted due to their scarcity and therefore DC models are 
      commonly employed in DC-based immunotherapy strategies and in vitro tests 
      assessing DC function. However, the interrelationship between the individual in 
      vitro DC models and their relative resemblance to specific primary DC populations 
      remain elusive. OBJECTIVE: To describe and assess functionality and applicability 
      of the available in vitro DC models by using a genome-wide transcriptional 
      approach. METHODS: Transcriptional profiling was performed with four commonly 
      used in vitro DC models (MUTZ-3-DCs, monocyte-derived DCs, CD34-derived DCs and 
      Langerhans cells (LCs)) and nine primary DC populations (dermal DCs, LCs, blood 
      and tonsillar CD123(+), CD1c(+) and CD141(+) DCs, and blood CD16(+) DCs). 
      RESULTS: Principal Component Analysis showed that transcriptional profiles of 
      each in vitro DC model most closely resembled CD1c(+) and CD141(+) tonsillar 
      myeloid DCs (mDCs) among primary DC populations. Thus, additional differentiation 
      factors may be required to generate model DCs that more closely resemble other 
      primary DC populations. Also, no model DC stood out in terms of primary DC 
      resemblance. Nevertheless, hierarchical clustering showed clusters of 
      differentially expressed genes among individual DC models as well as primary DC 
      populations. Furthermore, model DCs were shown to differentially express 
      immunologically relevant transcripts and transcriptional signatures identified 
      for each model DC included several immune-associated transcripts. CONCLUSION: The 
      unique transcriptional profiles of in vitro DC models suggest distinct 
      functionality in immune applications. The presented results will aid in the 
      selection of an appropriate DC model for in vitro assays and assist development 
      of DC-based immunotherapy.
FAU - Lundberg, Kristina
AU  - Lundberg K
AD  - Department of Immunotechnology, Lund University, Lund, Sweden. 
      kristina.lundberg@immun.lth.se
FAU - Albrekt, Ann-Sofie
AU  - Albrekt AS
FAU - Nelissen, Inge
AU  - Nelissen I
FAU - Santegoets, Saskia
AU  - Santegoets S
FAU - de Gruijl, Tanja D
AU  - de Gruijl TD
FAU - Gibbs, Sue
AU  - Gibbs S
FAU - Lindstedt, Malin
AU  - Lindstedt M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130114
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cluster Analysis
MH  - Dendritic Cells/*cytology/*immunology
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immune System Diseases/therapy
MH  - Immunologic Tests
MH  - *Models, Immunological
MH  - Palatine Tonsil/cytology
MH  - Phenotype
MH  - RNA, Messenger/genetics/metabolism
MH  - Skin/cytology
PMC - PMC3544800
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/01/24 06:00
MHDA- 2013/07/10 06:00
PMCR- 2013/01/14
CRDT- 2013/01/24 06:00
PHST- 2012/09/04 00:00 [received]
PHST- 2012/11/22 00:00 [accepted]
PHST- 2013/01/24 06:00 [entrez]
PHST- 2013/01/24 06:00 [pubmed]
PHST- 2013/07/10 06:00 [medline]
PHST- 2013/01/14 00:00 [pmc-release]
AID - PONE-D-12-26955 [pii]
AID - 10.1371/journal.pone.0052875 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(1):e52875. doi: 10.1371/journal.pone.0052875. Epub 2013 Jan 14.