PMID- 23342177
OWN - NLM
STAT- MEDLINE
DCOM- 20130619
LR  - 20211021
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Print)
IS  - 1935-2727 (Linking)
VI  - 7
IP  - 1
DP  - 2013
TI  - Comparison of the pathogenicity of Nipah virus isolates from Bangladesh and 
      Malaysia in the Syrian hamster.
PG  - e2024
LID - 10.1371/journal.pntd.0002024 [doi]
LID - e2024
AB  - Nipah virus is a zoonotic pathogen that causes severe disease in humans. The 
      mechanisms of pathogenesis are not well described. The first Nipah virus outbreak 
      occurred in Malaysia, where human disease had a strong neurological component. 
      Subsequent outbreaks have occurred in Bangladesh and India and transmission and 
      disease processes in these outbreaks appear to be different from those of the 
      Malaysian outbreak. Until this point, virtually all Nipah virus studies in vitro 
      and in vivo, including vaccine and pathogenesis studies, have utilized a virus 
      isolate from the original Malaysian outbreak (NiV-M). To investigate potential 
      differences between NiV-M and a Nipah virus isolate from Bangladesh (NiV-B), we 
      compared NiV-M and NiV-B infection in vitro and in vivo. In hamster kidney cells, 
      NiV-M-infection resulted in extensive syncytia formation and cytopathic effects, 
      whereas NiV-B-infection resulted in little to no morphological changes. In vivo, 
      NiV-M-infected Syrian hamsters had accelerated virus replication, pathology and 
      death when compared to NiV-B-infected animals. NiV-M infection also resulted in 
      the activation of host immune response genes at an earlier time point. 
      Pathogenicity was not only a result of direct effects of virus replication, but 
      likely also had an immunopathogenic component. The differences observed between 
      NiV-M and NiV-B pathogeneis in hamsters may relate to differences observed in 
      human cases. Characterization of the hamster model for NiV-B infection allows for 
      further research of the strain of Nipah virus responsible for the more recent 
      outbreaks in humans. This model can be used to study NiV-B pathogenesis, 
      transmission, and countermeasures that could be used to control outbreaks.
FAU - DeBuysscher, Blair L
AU  - DeBuysscher BL
AD  - Laboratory of Virology, Division of Intramural Research, National Institute of 
      Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain 
      Laboratories, Hamilton, Montana, USA.
FAU - de Wit, Emmie
AU  - de Wit E
FAU - Munster, Vincent J
AU  - Munster VJ
FAU - Scott, Dana
AU  - Scott D
FAU - Feldmann, Heinz
AU  - Feldmann H
FAU - Prescott, Joseph
AU  - Prescott J
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20130117
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
SB  - IM
MH  - Animals
MH  - Bangladesh
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Cytopathogenic Effect, Viral
MH  - Disease Models, Animal
MH  - Female
MH  - Giant Cells/virology
MH  - Henipavirus Infections/*pathology/*virology
MH  - Humans
MH  - Malaysia
MH  - Mesocricetus
MH  - Nipah Virus/*isolation & purification/*pathogenicity
MH  - Survival Analysis
MH  - Time Factors
MH  - Virus Cultivation
PMC - PMC3547834
COIS- The authors have declared that no competing interests exist.
EDAT- 2013/01/24 06:00
MHDA- 2013/06/20 06:00
PMCR- 2013/01/17
CRDT- 2013/01/24 06:00
PHST- 2012/10/10 00:00 [received]
PHST- 2012/12/05 00:00 [accepted]
PHST- 2013/01/24 06:00 [entrez]
PHST- 2013/01/24 06:00 [pubmed]
PHST- 2013/06/20 06:00 [medline]
PHST- 2013/01/17 00:00 [pmc-release]
AID - PNTD-D-12-01284 [pii]
AID - 10.1371/journal.pntd.0002024 [doi]
PST - ppublish
SO  - PLoS Negl Trop Dis. 2013;7(1):e2024. doi: 10.1371/journal.pntd.0002024. Epub 2013 
      Jan 17.