PMID- 23342772
OWN - NLM
STAT- MEDLINE
DCOM- 20130226
LR  - 20161020
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 37
IP  - 5
DP  - 2012 Oct
TI  - [Effect of electroacupuncture at different acupoints on expression of 
      cervico-spinal GDNF and BDNF and their receptor genes in neck-incision pain 
      rats].
PG  - 351-6
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Futu" (LI 18), 
      etc. on the expression of genes of glial cell line-derived neurotrophic factor 
      (GDNF), brain-derived neurotrophic factor (BDNF) and their receptors in the 
      cervico-spinal cord in neck-incision pain rats, so as to study its mechanism 
      underlying incision-pain relief. METHODS: A total of 50 Sprague Dawley male rats 
      were randomly divided into normal, model (incision pain), EA-Futu (LI 18), EA- 
      Hegu (LI 4) - Neiguan (PC 6, LI 4-PC 6) and EA-Zusanli (ST 36)-Yanglingquan (GB 
      34, ST 36-GB 34) groups (n = 10/group). A 1.5 cm long longitudinal incision was 
      made along the midline of the neck under anesthesia to duplicate neck-incision 
      pain model. Thermal pain threshold (PT) was measured before and after modeling 
      and after the treatment, respectively. EA (1-2 mA, 2 Hz/100 Hz) was applied to 
      bilateral LI 18, PC 6-LI 4 and ST 36-GB 34 for 30 min. The expression of genes of 
      GDNF and its receptor GFRalpha-1, and BDNF and its receptors TrkA, TrkB in the 
      spinal cord (C1 - C4) tissue was detected by quantitative real-time-PCR. RESULTS: 
      In comparison with pre-modeling in the same one group, the thermal PT levels were 
      decreased obviously in the model, LI 18, LI 4-PC 6 and ST 36-GB 34 groups after 
      neck incision (P < 0.05). Compared with the model group, the PT levels were 
      increased markedly in LI 18, LI 4-PC 6 and ST 36-GB 34 groups after the EA 
      treatment (P < 0.05). Correspondingly, the expression levels of spinal GDNF mRNA 
      and GFRalpha-1 mRNA were obviously lower, as well as BDNF mRNA was markedly 
      higher in the model group than in the control group (P < 0.05), and those of TrkA 
      mRNA and TrkB mRNA were increased slightly in the model group than in the control 
      group (P > 0.05). In comparison with the model group, the expression levels of 
      spinal GDNF mRNA and GFRalpha-1 mRNA were up-regulated considerably (P < 0.001), 
      whereas those of BDNF mRNA, TrkA mRNA and TrkB mRNA were decreased slightly (P > 
      0.05) in the LI 18, LI 4-PC 6 and ST 36-GB 34 groups after EA treatment. 
      CONCLUSION: EA stimulation can significantly suppress pain reaction of neck 
      incision, which is closely associated with its effects in up-regulating the 
      expression of GDNF and its receptor GFRalpha-1 genes in the cervical spinal cord 
      (C1 - C4).
FAU - Wang, Shao-Jun
AU  - Wang SJ
AD  - Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Tan, Lian-Hong
AU  - Tan LH
FAU - Liu, Jun-Ling
AU  - Liu JL
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - *Acupuncture Points
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - *Electroacupuncture
MH  - Gene Expression
MH  - Glial Cell Line-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Glial Cell Line-Derived Neurotrophic Factor Receptors/*genetics/metabolism
MH  - Humans
MH  - Male
MH  - Neck Pain/*genetics/metabolism/surgery/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*genetics/metabolism
MH  - Spinal Cord/metabolism
EDAT- 2013/01/25 06:00
MHDA- 2013/02/27 06:00
CRDT- 2013/01/25 06:00
PHST- 2013/01/25 06:00 [entrez]
PHST- 2013/01/25 06:00 [pubmed]
PHST- 2013/02/27 06:00 [medline]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2012 Oct;37(5):351-6.