PMID- 23344021
OWN - NLM
STAT- MEDLINE
DCOM- 20150831
LR  - 20211021
IS  - 1422-0067 (Print)
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 1
DP  - 2012 Dec 20
TI  - Chromosome-specific DNA repeats: rapid identification in silico and validation 
      using fluorescence in situ hybridization.
PG  - 57-71
LID - 10.3390/ijms14010057 [doi]
AB  - Chromosome enumeration in interphase and metaphase cells using fluorescence in 
      situ hybridization (FISH) is an established procedure for the rapid and accurate 
      cytogenetic analysis of cell nuclei and polar bodies, the unambiguous gender 
      determination, as well as the definition of tumor-specific signatures. Present 
      bottlenecks in the procedure are a limited number of commercial, non-isotopically 
      labeled probes that can be combined in multiplex FISH assays and the relatively 
      high price and effort to develop additional probes. We describe a streamlined 
      approach for rapid probe definition, synthesis and validation, which is based on 
      the analysis of publicly available DNA sequence information, also known as 
      "database mining". Examples of probe preparation for the human gonosomes and 
      chromosome 16 as a selected autosome outline the probe selection strategy, define 
      a timeline for expedited probe production and compare this novel selection 
      strategy to more conventional probe cloning protocols.
FAU - Hsu, Joanne H
AU  - Hsu JH
AD  - Department of Molecular and Cell Biology, University of California, Berkeley 
      (UCB), Berkeley, CA 94720, USA. jhhsu@berkeley.edu.
FAU - Zeng, Hui
AU  - Zeng H
FAU - Lemke, Kalistyn H
AU  - Lemke KH
FAU - Polyzos, Aris A
AU  - Polyzos AA
FAU - Weier, Jingly F
AU  - Weier JF
FAU - Wang, Mei
AU  - Wang M
FAU - Lawin-O'Brien, Anna R
AU  - Lawin-O'Brien AR
FAU - Weier, Heinz-Ulrich G
AU  - Weier HU
FAU - O'Brien, Benjamin
AU  - O'Brien B
LA  - eng
GR  - R01 CA136685/CA/NCI NIH HHS/United States
GR  - R01 HD045736/HD/NICHD NIH HHS/United States
GR  - R21 CA123370/CA/NCI NIH HHS/United States
GR  - R21 CA132815/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20121220
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - Clone Cells
MH  - *Computer Simulation
MH  - DNA Probes/metabolism
MH  - DNA, Satellite/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Reproducibility of Results
MH  - Sex Chromosomes/genetics
MH  - Trisomy/genetics
PMC - PMC3565251
EDAT- 2013/01/25 06:00
MHDA- 2013/01/25 06:01
PMCR- 2013/01/01
CRDT- 2013/01/25 06:00
PHST- 2012/12/02 00:00 [received]
PHST- 2012/12/09 00:00 [revised]
PHST- 2012/12/10 00:00 [accepted]
PHST- 2013/01/25 06:00 [entrez]
PHST- 2013/01/25 06:00 [pubmed]
PHST- 2013/01/25 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - ijms14010057 [pii]
AID - ijms-14-00057 [pii]
AID - 10.3390/ijms14010057 [doi]
PST - epublish
SO  - Int J Mol Sci. 2012 Dec 20;14(1):57-71. doi: 10.3390/ijms14010057.