PMID- 23347260
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR  - 20130415
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 37
IP  - 8
DP  - 2013 Apr
TI  - Differential effects of site-specific knockdown of estrogen receptor alpha in the 
      medial amygdala, medial pre-optic area, and ventromedial nucleus of the 
      hypothalamus on sexual and aggressive behavior of male mice.
PG  - 1308-19
LID - 10.1111/ejn.12131 [doi]
AB  - Testosterone is known to play an important role in the regulation of male-type 
      sexual and aggressive behavior. As an aromatised metabolite of testosterone, 
      estradiol-induced activation of estrogen receptor alpha (ERalpha) may be crucial for the 
      induction of these behaviors in male mice. However, the importance of ERalpha 
      expressed in different nuclei for this facilitatory action of testosterone has 
      not been determined. To investigate this issue, we generated an adeno-associated 
      virus vector expressing a small hairpin RNA targeting ERalpha to site-specifically 
      knockdown ERalpha expression. We stereotaxically injected either a control or ERalpha 
      targeting vector into the medial amygdala, medial pre-optic area (MPOA), or 
      ventromedial nucleus of the hypothalamus (VMN) in gonadally intact male mice. Two 
      weeks after injection, all mice were tested biweekly for sexual and aggressive 
      behavior, alternating between behavior tests each week. We found that suppressing 
      ERalpha in the MPOA reduced sexual but not aggressive behavior, whereas in the VMN it 
      reduced both behaviors. Knockdown of ERalpha in the medial amygdala did not alter 
      either behavior. Additionally, it was found that ERalpha knockdown in the MPOA caused 
      a parallel reduction in the number of neuronal nitric oxide synthase-expressing 
      cells. Taken together, these results indicate that the testosterone facilitatory 
      action on male sexual behavior requires the expression of ERalpha in both the MPOA 
      and VMN, whereas the testosterone facilitatory action on aggression requires the 
      expression of ERalpha in only the VMN.
CI  - (c) 2013 Federation of European Neuroscience Societies and Blackwell Publishing 
      Ltd.
FAU - Sano, Kazuhiro
AU  - Sano K
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, Tsukuba, 
      Ibaraki, Japan.
FAU - Tsuda, Mumeko C
AU  - Tsuda MC
FAU - Musatov, Sergei
AU  - Musatov S
FAU - Sakamoto, Toshiro
AU  - Sakamoto T
FAU - Ogawa, Sonoko
AU  - Ogawa S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130124
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Aggression/*physiology
MH  - Animals
MH  - Brain/*metabolism
MH  - Estrogen Receptor alpha/*metabolism
MH  - Gene Knockdown Techniques
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - RNA, Small Interfering
MH  - Sexual Behavior, Animal/*physiology
EDAT- 2013/01/26 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/01/26 06:00
PHST- 2012/09/19 00:00 [received]
PHST- 2012/12/11 00:00 [revised]
PHST- 2012/12/17 00:00 [accepted]
PHST- 2013/01/26 06:00 [entrez]
PHST- 2013/01/26 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - 10.1111/ejn.12131 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2013 Apr;37(8):1308-19. doi: 10.1111/ejn.12131. Epub 2013 Jan 24.