PMID- 23349717
OWN - NLM
STAT- MEDLINE
DCOM- 20130709
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 1
DP  - 2013
TI  - Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the 
      effect of sex hormones.
PG  - e53562
LID - 10.1371/journal.pone.0053562 [doi]
LID - e53562
AB  - The liver is one of the most sex-dimorphic organs in both oviparous and 
      viviparous animals. In order to understand the molecular basis of the difference 
      between male and female livers, high-throughput RNA-SAGE (serial analysis of gene 
      expression) sequencing was performed for zebrafish livers of both sexes and their 
      transcriptomes were compared. Both sexes had abundantly expressed genes involved 
      in translation, coagulation and lipid metabolism, consistent with the general 
      function of the liver. For sex-biased transcripts, from in addition to the high 
      enrichment of vitellogenin transcripts in spawning female livers, which 
      constituted nearly 80% of total mRNA, it is apparent that the female-biased genes 
      were mostly involved in ribosome/translation, estrogen pathway, lipid transport, 
      etc, while the male-biased genes were enriched for oxidation reduction, 
      carbohydrate metabolism, coagulation, protein transport and localization, etc. 
      Sexual dimorphism on xenobiotic metabolism and anti-oxidation was also noted and 
      it is likely that retinol x receptor (RXR) and liver x receptor (LXR) play 
      central roles in regulating the sexual differences of lipid and cholesterol 
      metabolisms. Consistent with high ribosomal/translational activities in the 
      female liver, female-biased genes were significantly regulated by two important 
      transcription factors, Myc and Mycn. In contrast, Male livers showed activation 
      of transcription factors Ppargc1b, Hnf4a, and Stat4, which regulate lipid and 
      glucose metabolisms and various cellular activities. The transcriptomic responses 
      to sex hormones, 17beta-estradiol (E2) or 11-keto testosterone (KT11), were also 
      investigated in both male and female livers and we found that female livers were 
      relatively insensitive to sex hormone disturbance, while the male livers were 
      readily affected. E2 feminized male liver by up-regulating female-biased 
      transcripts and down-regulating male-biased transcripts. The information obtained 
      in this study provides comprehensive insights into the sexual dimorphism of 
      zebrafish liver transcriptome and will facilitate further development of the 
      zebrafish as a human liver disease model.
FAU - Zheng, Weiling
AU  - Zheng W
AD  - Department of Biological Sciences, National University of Singapore, Singapore, 
      Singapore.
FAU - Xu, Hongyan
AU  - Xu H
FAU - Lam, Siew Hong
AU  - Lam SH
FAU - Luo, Huaien
AU  - Luo H
FAU - Karuturi, R Krishna Murthy
AU  - Karuturi RK
FAU - Gong, Zhiyuan
AU  - Gong Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130117
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Xenobiotics)
SB  - IM
MH  - Animals
MH  - Female
MH  - *Gene Expression Profiling
MH  - Gene Regulatory Networks/drug effects
MH  - Gonadal Steroid Hormones/*pharmacology
MH  - Humans
MH  - Liver/*drug effects/*metabolism
MH  - Male
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Analysis, RNA
MH  - *Sex Characteristics
MH  - Xenobiotics/metabolism
MH  - Zebrafish/*genetics/metabolism
PMC - PMC3547925
COIS- Competing Interests: Co-author Zhiyuan Gongis is a PLOS ONE Editorial Board 
      member. This does not alter the authors' adherence to all the PLOS ONE policies 
      on sharing data and materials.
EDAT- 2013/01/26 06:00
MHDA- 2013/07/10 06:00
PMCR- 2013/01/17
CRDT- 2013/01/26 06:00
PHST- 2012/09/09 00:00 [received]
PHST- 2012/11/29 00:00 [accepted]
PHST- 2013/01/26 06:00 [entrez]
PHST- 2013/01/26 06:00 [pubmed]
PHST- 2013/07/10 06:00 [medline]
PHST- 2013/01/17 00:00 [pmc-release]
AID - PONE-D-12-27355 [pii]
AID - 10.1371/journal.pone.0053562 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(1):e53562. doi: 10.1371/journal.pone.0053562. Epub 2013 Jan 17.