PMID- 23349788
OWN - NLM
STAT- MEDLINE
DCOM- 20130724
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 1
DP  - 2013
TI  - Activation of thromboxane A2 receptor (TP) increases the expression of monocyte 
      chemoattractant protein -1 (MCP-1)/chemokine (C-C motif) ligand 2 (CCL2) and 
      recruits macrophages to promote invasion of lung cancer cells.
PG  - e54073
LID - 10.1371/journal.pone.0054073 [doi]
LID - e54073
AB  - Thromboxane synthase (TXAS) and thromboxane A(2) receptor (TP), two critical 
      components for thromboxane A(2) (TXA(2)) signaling, have been suggested to be 
      involved in cancer invasion and metastasis. However, the mechanisms by which 
      TXA(2) promotes these processes are still unclear. Here we show that TXA(2) 
      mimetic, I-BOP, induced monocyte chemoattractant protein -1(MCP-1)/chemokine (C-C 
      motif) ligand 2 (CCL2) expression at both mRNA and protein levels in human lung 
      adenocarcinoma A549 cells stably over-expressing TP receptor alpha isoform 
      (A549-TPalpha). The induction of MCP-1 was also found in other lung cancer cells H157 
      and H460 that express relatively high levels of endogenous TP. Using specific 
      inhibitors of several signaling molecules and promoter/luciferase assay, we 
      identified that transcription factor SP1 mediates I-BOP-induced MCP-1 expression. 
      Furthermore, supernatants from I-BOP-treated A549-TPalpha cells enhanced 
      MCP-1-dependent migration of RAW 264.7 macrophages. Moreover, co-culture of A549 
      cells with RAW 264.7 macrophages induced expression of MMPs, VEGF and MCP-1 
      genes, and increased the invasive potential in A549 cells. These findings suggest 
      that TXA(2) may stimulate invasion of cancer cells through MCP-1-mediated 
      macrophage recruitment.
FAU - Li, Xiuling
AU  - Li X
AD  - Department of Pharmaceutical Sciences, College of Pharmacy, University of 
      Kentucky, Lexington, Kentucky, USA.
FAU - Tai, Hsin-Hsiung
AU  - Tai HH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130117
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Receptors, Thromboxane A2, Prostaglandin H2)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 124924-85-2 
      (7-(3-(3-hydroxy-4-(4'-iodophenoxy)-1-butenyl)-7-oxabicyclo(2.2.1)heptan-2-yl)-5-heptenoic 
      acid)
RN  - 57576-52-0 (Thromboxane A2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Coculture Techniques
MH  - Fatty Acids, Unsaturated/pharmacology
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Lung Neoplasms/genetics/metabolism/pathology
MH  - Macrophages/cytology/*metabolism
MH  - Matrix Metalloproteinases/genetics/metabolism
MH  - Microscopy, Fluorescence
MH  - Neoplasm Invasiveness
MH  - Receptors, Thromboxane A2, Prostaglandin H2/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sp1 Transcription Factor/metabolism
MH  - Thromboxane A2/pharmacology
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
PMC - PMC3547941
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/01/26 06:00
MHDA- 2013/07/25 06:00
PMCR- 2013/01/17
CRDT- 2013/01/26 06:00
PHST- 2012/05/29 00:00 [received]
PHST- 2012/12/06 00:00 [accepted]
PHST- 2013/01/26 06:00 [entrez]
PHST- 2013/01/26 06:00 [pubmed]
PHST- 2013/07/25 06:00 [medline]
PHST- 2013/01/17 00:00 [pmc-release]
AID - PONE-D-12-15041 [pii]
AID - 10.1371/journal.pone.0054073 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(1):e54073. doi: 10.1371/journal.pone.0054073. Epub 2013 Jan 17.