PMID- 23350932
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20130225
IS  - 1365-2443 (Electronic)
IS  - 1356-9597 (Linking)
VI  - 18
IP  - 3
DP  - 2013 Mar
TI  - TAF4b and TAF4 differentially regulate mouse embryonic stem cells maintenance and 
      proliferation.
PG  - 225-37
LID - 10.1111/gtc.12030 [doi]
AB  - TAF4b is a cell type-specific subunit of the general transcription factor TFIID. 
      Here, we show that TAF4b is highly expressed in embryonic stem cells (ESC) and is 
      down-regulated upon differentiation. To examine the role of TAF4b in ESC, we 
      applied a knockdown (KD) approach. TAF4b depletion is associated with 
      morphological changes and reduced expression of the self-renewal marker alkaline 
      phosphatase. In contrast, KD of TAF4, a ubiquitously expressed TAF4b paralog, 
      retained and even stabilized ESC stemness. Retinoic acid-induced differentiation 
      was facilitated in the absence of TAF4b but was significantly delayed by TAF4 KD. 
      Furthermore, TAF4b supports, whereas TAF4 inhibits, ESC proliferation and cell 
      cycle progression. We identified a subset of TAF4b target genes preferentially 
      expressed in ESC and controlling the cell cycle. Among them are the germ 
      cell-specific transcription factor Sohlh2 and the protein kinase Yes1, which was 
      recently shown to regulate ESC self-renewal. Interestingly, Sohlh2 and Yes1 are 
      also targets of the pluripotency factor Oct4, and their regulation by Oct4 is 
      TAF4b-dependent. Consistent with that, TAF4b but not TAF4 interacts with Oct4. 
      Our findings suggest that TAF4b cooperates with Oct4 to regulate a subset of 
      genes in ESC, whereas TAF4 is required for later embryonic developmental stages.
CI  - (c) 2013 The Authors Genes to Cells (c) 2013 by the Molecular Biology Society of 
      Japan and Wiley Publishing Asia Pty Ltd.
FAU - Bahat, Anat
AU  - Bahat A
AD  - Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 
      76100, Israel.
FAU - Kedmi, Ranit
AU  - Kedmi R
FAU - Gazit, Kfir
AU  - Gazit K
FAU - Richardo-Lax, Inna
AU  - Richardo-Lax I
FAU - Ainbinder, Elena
AU  - Ainbinder E
FAU - Dikstein, Rivka
AU  - Dikstein R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130127
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (Pou5f1 protein, mouse)
RN  - 0 (Sohlh2 protein, mouse)
RN  - 0 (TAF4 protein, mouse)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (Taf4b protein, mouse)
RN  - 0 (Transcription Factor TFIID)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-yes)
RN  - EC 2.7.10.2 (Yes1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
MH  - Cell Cycle/genetics
MH  - Cell Line
MH  - *Cell Proliferation
MH  - Embryonic Stem Cells/cytology/*metabolism
MH  - *Gene Expression Regulation
MH  - Mice
MH  - Mutation
MH  - Octamer Transcription Factor-3/genetics/metabolism
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-yes/genetics/metabolism
MH  - TATA-Binding Protein Associated Factors/genetics/*metabolism
MH  - Transcription Factor TFIID/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 2013/01/29 06:00
MHDA- 2013/08/06 06:00
CRDT- 2013/01/29 06:00
PHST- 2012/03/13 00:00 [received]
PHST- 2012/12/05 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
AID - 10.1111/gtc.12030 [doi]
PST - ppublish
SO  - Genes Cells. 2013 Mar;18(3):225-37. doi: 10.1111/gtc.12030. Epub 2013 Jan 27.