PMID- 23352172
OWN - NLM
STAT- MEDLINE
DCOM- 20130829
LR  - 20130307
IS  - 1877-9603 (Electronic)
IS  - 1877-959X (Linking)
VI  - 4
IP  - 3
DP  - 2013 Apr
TI  - Inhibition of cell death of bone marrow-derived macrophages infected with 
      Ehrlichia muris.
PG  - 185-90
LID - S1877-959X(12)00147-1 [pii]
LID - 10.1016/j.ttbdis.2012.11.011 [doi]
AB  - Ehrlichia muris is a Gram-negative obligate intracellular bacterium belonging to 
      the family Anaplasmataceae. It preferentially replicates inside macrophages by 
      utilizing nutrients and processes of the host cell. In the present article, we 
      studied the effects of E. muris infection on cell death of bone marrow-derived 
      macrophages (BMDMs). Primary BMDMs were used for accessing E. muris-induced cell 
      death, pro-inflammatory cytokine production and Western blot analysis. Human 
      embryonic kidney cell line 293T (HEK293T) was used to access nuclear 
      factor-kappaB (NF-kappaB) activity. BMDMs infected with E. muris showed significant 
      inhibition of cell death when compared to uninfected cells. E. muris infection 
      resulted in IkappaBalpha degradation, thus activation of NF-kappaB. In NF-kappaB reporter gene 
      assay, the HEK293T cells infected with E. muris exhibited robust NF-kappaB-dependent 
      luciferase activity in a bacterial dose-dependent manner. Furthermore, E. 
      muris-induced inhibition of BMDMs cell death was abolished in the presence of 
      MG132, a proteasome inhibitor that blocks NF-kappaB activation. Taken together, the 
      results suggest that E. muris infection of BMDMs may have an inhibitory effect on 
      cell death via a mechanism dependent on NF-kappaB activation.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - Mathema, Vivek Bhakta
AU  - Mathema VB
AD  - School of Medicine, Jeju National University, Jeju 690-756, South Korea.
FAU - Manzoor, Zahid
AU  - Manzoor Z
FAU - Koo, Jung-Eun
AU  - Koo JE
FAU - Koh, Young-Sang
AU  - Koh YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121227
PL  - Netherlands
TA  - Ticks Tick Borne Dis
JT  - Ticks and tick-borne diseases
JID - 101522599
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Cell Death/*physiology
MH  - Ehrlichia/*physiology
MH  - Ehrlichiosis/pathology
MH  - Female
MH  - Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Macrophages/cytology/*microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
EDAT- 2013/01/29 06:00
MHDA- 2013/08/30 06:00
CRDT- 2013/01/29 06:00
PHST- 2012/03/27 00:00 [received]
PHST- 2012/11/14 00:00 [revised]
PHST- 2012/11/14 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/08/30 06:00 [medline]
AID - S1877-959X(12)00147-1 [pii]
AID - 10.1016/j.ttbdis.2012.11.011 [doi]
PST - ppublish
SO  - Ticks Tick Borne Dis. 2013 Apr;4(3):185-90. doi: 10.1016/j.ttbdis.2012.11.011. 
      Epub 2012 Dec 27.