PMID- 23352232
OWN - NLM
STAT- MEDLINE
DCOM- 20130412
LR  - 20201226
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 38
IP  - 2
DP  - 2013 Feb 21
TI  - Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain 
      T helper 2 cell-mediated immunity to house dust mite allergen.
PG  - 322-35
LID - S1074-7613(13)00004-6 [pii]
LID - 10.1016/j.immuni.2012.10.016 [doi]
AB  - Dendritic cells (DCs) are crucial for mounting allergic airway inflammation, but 
      it is unclear which subset of DCs performs this task. By using CD64 and MAR-1 
      staining, we reliably separated CD11b(+) monocyte-derived DCs (moDCs) from 
      conventional DCs (cDCs) and studied antigen uptake, migration, and presentation 
      assays of lung and lymph node (LN) DCs in response to inhaled house dust mite 
      (HDM). Mainly CD11b(+) cDCs but not CD103(+) cDCs induced T helper 2 (Th2) cell 
      immunity in HDM-specific T cells in vitro and asthma in vivo. Studies in 
      Flt3l(-/-) mice, lacking all cDCs, revealed that moDCs were also sufficient to 
      induce Th2 cell-mediated immunity but only when high-dose HDM was given. The main 
      function of moDCs was the production of proinflammatory chemokines and allergen 
      presentation in the lung during challenge. Thus, we have identified migratory 
      CD11b(+) cDCs as the principal subset inducing Th2 cell-mediated immunity in the 
      LN, whereas moDCs orchestrate allergic inflammation in the lung.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Plantinga, Maud
AU  - Plantinga M
AD  - Laboratory of Immunoregulation and Mucosal Immunology, Department for Molecular 
      Biomedical Research, VIB, Ghent 9050, Belgium.
FAU - Guilliams, Martin
AU  - Guilliams M
FAU - Vanheerswynghels, Manon
AU  - Vanheerswynghels M
FAU - Deswarte, Kim
AU  - Deswarte K
FAU - Branco-Madeira, Filipe
AU  - Branco-Madeira F
FAU - Toussaint, Wendy
AU  - Toussaint W
FAU - Vanhoutte, Leen
AU  - Vanhoutte L
FAU - Neyt, Katrijn
AU  - Neyt K
FAU - Killeen, Nigel
AU  - Killeen N
FAU - Malissen, Bernard
AU  - Malissen B
FAU - Hammad, Hamida
AU  - Hammad H
FAU - Lambrecht, Bart N
AU  - Lambrecht BN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130124
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Antigens, Ly)
RN  - 0 (CD11b Antigen)
RN  - 0 (Natural Cytotoxicity Triggering Receptor 1)
RN  - 0 (Ncr1 protein, mouse)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Administration, Inhalation
MH  - Adoptive Transfer
MH  - Allergens/*immunology/isolation & purification
MH  - Animals
MH  - Antigens, Dermatophagoides/administration & dosage/*immunology/isolation & 
      purification
MH  - Antigens, Ly/genetics/immunology
MH  - Asthma/*immunology/pathology
MH  - CD11b Antigen/genetics/immunology
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Dendritic Cells/*immunology/transplantation
MH  - Gene Expression
MH  - *Immunity, Cellular
MH  - Inflammation/immunology/pathology
MH  - Lung/immunology/pathology
MH  - Lymph Nodes/immunology/pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/immunology/transplantation
MH  - Natural Cytotoxicity Triggering Receptor 1/genetics/immunology
MH  - Organ Specificity
MH  - Pyroglyphidae/*immunology
MH  - Receptors, IgG/genetics/immunology
MH  - Th2 Cells/*immunology
EDAT- 2013/01/29 06:00
MHDA- 2013/04/13 06:00
CRDT- 2013/01/29 06:00
PHST- 2012/07/09 00:00 [received]
PHST- 2012/10/22 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/04/13 06:00 [medline]
AID - S1074-7613(13)00004-6 [pii]
AID - 10.1016/j.immuni.2012.10.016 [doi]
PST - ppublish
SO  - Immunity. 2013 Feb 21;38(2):322-35. doi: 10.1016/j.immuni.2012.10.016. Epub 2013 
      Jan 24.