PMID- 23352830
OWN - NLM
STAT- MEDLINE
DCOM- 20130819
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1292
DP  - 2013 May 31
TI  - Reversed-phase ion-pair ultra-high-performance-liquid chromatography-mass 
      spectrometry for fingerprinting low-molecular-weight heparins.
PG  - 201-10
LID - S0021-9673(13)00066-6 [pii]
LID - 10.1016/j.chroma.2013.01.011 [doi]
AB  - Heparin is a complex mixture of sulfated linear carbohydrate polymers. It is 
      widely used as an antithrombotic drug, though it has been shown to have a myriad 
      of additional biological activities. Heparin is often partially depolymerized in 
      order to decrease the average molecular weight, as it has been shown that low 
      molecular weight heparins (LMWH) possess more desirable pharmacokinetic and 
      pharmacodynamic properties than unfractionated heparin (UFH). Due to the 
      prevalence of LMWHs in the market and the emerging availability of generic LMWH 
      products, it is important that analytical methods be developed to ensure the drug 
      quality. This work explores the use of tributylamine (TrBA), dibutylamine (DBA), 
      and pentylamine (PTA) as ion-pairing reagents in conjunction with acetonitrile 
      and methanol modified mobile phases for reversed-phase ion-pairing 
      ultraperformance liquid chromatography coupled to mass spectrometry 
      (RPIP-UPLC-MS) for fingerprint analysis of LMWH preparations. RPIP-UPLC-MS 
      fingerprints are presented and compared for tinzaparinand enoxaparin.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Langeslay, Derek J
AU  - Langeslay DJ
AD  - Department of Chemistry, University of California - Riverside, Riverside, CA, 
      USA.
FAU - Urso, Elena
AU  - Urso E
FAU - Gardini, Cristina
AU  - Gardini C
FAU - Naggi, Annamaria
AU  - Naggi A
FAU - Torri, Giangiacomo
AU  - Torri G
FAU - Larive, Cynthia K
AU  - Larive CK
LA  - eng
GR  - CA138535/CA/NCI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130110
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anticoagulants)
RN  - 0 (Butylamines)
RN  - 0 (Enoxaparin)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 2194M2LA21 (dibutylamine)
RN  - 7UQ7X4Y489 (Tinzaparin)
RN  - C3TZB2W0R7 (tributylamine)
SB  - IM
MH  - Anticoagulants/*chemistry/isolation & purification
MH  - Butylamines/chemistry
MH  - Chromatography, High Pressure Liquid/methods
MH  - Chromatography, Reverse-Phase/*methods
MH  - Enoxaparin/*chemistry/isolation & purification
MH  - Heparin, Low-Molecular-Weight/*chemistry/isolation & purification
MH  - Mass Spectrometry/*methods
MH  - Sensitivity and Specificity
MH  - Tinzaparin
EDAT- 2013/01/29 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/01/29 06:00
PHST- 2012/07/26 00:00 [received]
PHST- 2012/12/13 00:00 [revised]
PHST- 2013/01/04 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0021-9673(13)00066-6 [pii]
AID - 10.1016/j.chroma.2013.01.011 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 May 31;1292:201-10. doi: 10.1016/j.chroma.2013.01.011. Epub 
      2013 Jan 10.