PMID- 23352841
OWN - NLM
STAT- MEDLINE
DCOM- 20130412
LR  - 20211021
IS  - 2210-7762 (Print)
VI  - 206
IP  - 1-2
DP  - 2013 Jan-Feb
TI  - A novel four-color fluorescence in situ hybridization assay for the detection of 
      TMPRSS2 and ERG rearrangements in prostate cancer.
PG  - 1-11
LID - S2210-7762(12)00279-7 [pii]
LID - 10.1016/j.cancergen.2012.12.004 [doi]
AB  - Since the identification of the TMPRSS2-ERG rearrangement as the most common 
      fusion event in prostate cancer, various methods have been developed to detect 
      this rearrangement and to study its prognostic significance. We report a novel 
      four-color fluorescence in situ hybridization (FISH) assay that detects not only 
      the typical TMPRSS2-ERG fusion but also alternative rearrangements of the TMPRSS2 
      or ERG gene. We validated this assay on fresh, frozen, or formalin-fixed 
      paraffin-embedded prostate cancer specimens, including cell lines, primary 
      prostate cancer tissues, xenograft tissues derived from metastatic prostate 
      cancer, and metastatic tissues from castration-resistant prostate cancer (CRPC) 
      patients. When compared with either reverse transcription-polymerase chain 
      reaction or the Gen-Probe method as the technical reference, analysis using the 
      four-color FISH assay demonstrated an analytical sensitivity of 94.5% (95% 
      confidence interval [CI] 0.80-0.99) and specificity of 100% (95% CI 0.89-1.00) 
      for detecting the TMPRSS2-ERG fusion. The TMPRSS2-ERG fusion was detected in 41% 
      and 43% of primary prostate cancer (n = 59) and CRPC tumors (n = 82), 
      respectively. Rearrangements other than the typical TMPRSS2-ERG fusion were 
      confirmed by karyotype analysis and found in 7% of primary cancer and 13% of CRPC 
      tumors. Successful karyotype analyses are reported for the first time on four of 
      the xenograft samples, complementing the FISH results. Analysis using the 
      four-color FISH assay provides sensitive detection of TMPRSS2 and ERG gene 
      rearrangements in prostate cancer.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Qu, Xiaoyu
AU  - Qu X
AD  - Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
FAU - Randhawa, Grace
AU  - Randhawa G
FAU - Friedman, Cynthia
AU  - Friedman C
FAU - O'Hara-Larrivee, Siobhan
AU  - O'Hara-Larrivee S
FAU - Kroeger, Kathleen
AU  - Kroeger K
FAU - Dumpit, Ruth
AU  - Dumpit R
FAU - True, Larry
AU  - True L
FAU - Vakar-Lopez, Funda
AU  - Vakar-Lopez F
FAU - Porter, Christopher
AU  - Porter C
FAU - Vessella, Robert
AU  - Vessella R
FAU - Nelson, Peter
AU  - Nelson P
FAU - Fang, Min
AU  - Fang M
LA  - eng
GR  - P01 CA085859/CA/NCI NIH HHS/United States
GR  - P50 CA097186/CA/NCI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Validation Study
DEP - 20130124
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
RN  - 0 (ERG protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TMPRSS2-ERG fusion protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcriptional Regulator ERG)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (TMPRSS2 protein, human)
SB  - IM
MH  - Animals
MH  - Carcinoma/diagnosis/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Color
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Neoplasm Metastasis
MH  - Oncogene Proteins, Fusion/*analysis/genetics
MH  - Prostatic Neoplasms/diagnosis/*genetics/pathology
MH  - Sensitivity and Specificity
MH  - Serine Endopeptidases/analysis/*genetics
MH  - Trans-Activators/analysis/*genetics
MH  - Transcriptional Regulator ERG
MH  - Translocation, Genetic/genetics
MH  - Transplantation, Heterologous
PMC - PMC3632390
MID - NIHMS429656
EDAT- 2013/01/29 06:00
MHDA- 2013/04/13 06:00
PMCR- 2014/01/24
CRDT- 2013/01/29 06:00
PHST- 2012/11/02 00:00 [received]
PHST- 2012/12/11 00:00 [revised]
PHST- 2012/12/12 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/04/13 06:00 [medline]
PHST- 2014/01/24 00:00 [pmc-release]
AID - S2210-7762(12)00279-7 [pii]
AID - 10.1016/j.cancergen.2012.12.004 [doi]
PST - ppublish
SO  - Cancer Genet. 2013 Jan-Feb;206(1-2):1-11. doi: 10.1016/j.cancergen.2012.12.004. 
      Epub 2013 Jan 24.