PMID- 23352984 OWN - NLM STAT- MEDLINE DCOM- 20140210 LR - 20220330 IS - 1873-2747 (Electronic) IS - 0361-9230 (Linking) VI - 95 DP - 2013 Jun TI - G-protein-coupled receptor kinase interactor-1 serine 419 accelerates premature synapse formation in cortical neurons by interacting with Ca(2+)/calmodulin-dependent protein kinase IIbeta. PG - 70-7 LID - S0361-9230(13)00007-5 [pii] LID - 10.1016/j.brainresbull.2013.01.006 [doi] AB - In the present study, we investigated the mechanisms of brain derived neurotrophic factor (BDNF) in regulating cortical neuron premature synapse formation. KN-93, a specific inhibitor of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), and G-protein-coupled receptor kinase interactor-1 (G1T1) siRNA were utilized, and the premature synapse formation of cortical neurons was detected under BDNF stimulation. Plasmids HA-GIT1, HA-GIT1 (DeltaSLD), HA-GIT1 (S419A) and Flag-CaMKIIbeta were constructed. The interaction between GIT1 and CaMKIIbeta, and their influence on the premature synapse formation of BDNF-stimulated cortical neurons were examined. BDNF-stimulated cortical neurons were associated with increased premature synapse formation, the enhancement of phosphorylation for CaMKIIbeta, and the combination of GIT1 and p-CaMKII(thr286). G1T1 siRNA and KN-93 inhibited premature synapse formation in cortical neurons. The interaction between GIT1 and CaMKIIbeta required SLD domain and serine 419 in GIT1. BDNF-induced CaMKIIbeta phosphorylation and premature synapse formation were suppressed in GIT1 (S419A) transfected cortical neurons. By interacting with CaMKIIbeta, G1T1 (S419) were shown to participate in BDNF-induced premature synapse formation within cortical neurons. CI - Copyright (c) 2013 Elsevier Inc. All rights reserved. FAU - Zhang, Zitao AU - Zhang Z AD - Department of Spine Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. FAU - Zhou, Wei AU - Zhou W FAU - Fan, Jin AU - Fan J FAU - Ren, Yongxin AU - Ren Y FAU - Yin, Guoyong AU - Yin G LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130124 PL - United States TA - Brain Res Bull JT - Brain research bulletin JID - 7605818 RN - 0 (Benzylamines) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Calmodulin) RN - 0 (Cell Cycle Proteins) RN - 0 (Git1 protein, rat) RN - 0 (Phosphoproteins) RN - 0 (Sulfonamides) RN - 139298-40-1 (KN 93) RN - 452VLY9402 (Serine) RN - EC 2.7.11.16 (G-Protein-Coupled Receptor Kinases) RN - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2) SB - IM MH - Animals MH - Benzylamines MH - Brain-Derived Neurotrophic Factor/metabolism MH - Calcium-Calmodulin-Dependent Protein Kinase Type 2/*metabolism MH - Calmodulin/*metabolism MH - Cell Cycle Proteins/*metabolism MH - Cells, Cultured MH - G-Protein-Coupled Receptor Kinases/*antagonists & inhibitors MH - Neurons/metabolism MH - Phosphoproteins/*metabolism MH - Rats MH - Serine/metabolism MH - Signal Transduction/physiology MH - Sulfonamides MH - Synapses/*metabolism EDAT- 2013/01/29 06:00 MHDA- 2014/02/11 06:00 CRDT- 2013/01/29 06:00 PHST- 2012/07/24 00:00 [received] PHST- 2013/01/01 00:00 [revised] PHST- 2013/01/16 00:00 [accepted] PHST- 2013/01/29 06:00 [entrez] PHST- 2013/01/29 06:00 [pubmed] PHST- 2014/02/11 06:00 [medline] AID - S0361-9230(13)00007-5 [pii] AID - 10.1016/j.brainresbull.2013.01.006 [doi] PST - ppublish SO - Brain Res Bull. 2013 Jun;95:70-7. doi: 10.1016/j.brainresbull.2013.01.006. Epub 2013 Jan 24.