PMID- 23353778
OWN - NLM
STAT- MEDLINE
DCOM- 20131112
LR  - 20181202
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 173
IP  - 2
DP  - 2013 May
TI  - Development of a DNA-launched replicon as a vaccine for porcine reproductive and 
      respiratory syndrome virus.
PG  - 321-6
LID - S0168-1702(13)00012-9 [pii]
LID - 10.1016/j.virusres.2013.01.011 [doi]
AB  - Though a modified live attenuated vaccine (MLV) is available against porcine 
      reproductive and respiratory syndrome virus (PRRSV), its limitations in 
      protective efficacy, safety and few others warrant the development of newer 
      vaccines. In this study, we have constructed a propagation-defective DNA-launched 
      PRRSV replicon as a vaccine candidate and evaluated its immunogenicity and 
      protective efficacy in a group of pigs along with MLV vaccinated group. Our data 
      showed that prior to the intranasal challenge with a homologous strain of PRRSV, 
      only MLV vaccinated pigs developed antibody response measured by ELISA and none 
      of the pigs in any group developed PRRSV neutralizing antibodies in serum. The 
      MLV vaccinated group also showed high PRRSV-specific INF-gamma response, whereas the 
      replicon-vaccinated pigs showed low but detectable INF-gamma response. After 14 days 
      post challenge, all groups showed similar PRRSV-specific serum neutralizing 
      titers and were positive for PRRSV-specific ELISA antibody. In addition, the 
      replicon-vaccinated group showed a significant reduction in viremia in comparison 
      to the control group. In conclusion, vaccination with the PRRSV DNA-launched 
      replicon decreased the viremia and viral load in bronchoalveolar lavage fluids of 
      the PRRSV-challenged pigs and increased numbers of IFN-gamma producing cells. Thus, 
      the vaccine is partially protective and is a potential vaccine candidate for 
      future with further improvement. The possible means of improvement is the 
      expression of immunostimulatory genes by the replicon. We demonstrated the 
      feasibility of this approach by expression of a foreign gene encoding firefly 
      luciferase after transfection of cultured cells with the replicon plasmid DNA.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Pujhari, Sujit
AU  - Pujhari S
AD  - Vaccine and Infectious Disease Organization - International Vaccine Center 
      (VIDO-InterVac), University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK 
      S7N 5E3, Canada.
FAU - Baig, Tayyba T
AU  - Baig TT
FAU - Hansra, Satynder
AU  - Hansra S
FAU - Zakhartchouk, Alexander N
AU  - Zakhartchouk AN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130123
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Vaccines, Synthetic)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gammaretrovirus
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/genetics/immunology
MH  - Vaccines, DNA/administration & dosage/genetics/*immunology
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology
MH  - Viral Load
MH  - Viremia/prevention & control
EDAT- 2013/01/29 06:00
MHDA- 2013/11/13 06:00
CRDT- 2013/01/29 06:00
PHST- 2012/10/11 00:00 [received]
PHST- 2012/12/20 00:00 [revised]
PHST- 2013/01/14 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/11/13 06:00 [medline]
AID - S0168-1702(13)00012-9 [pii]
AID - 10.1016/j.virusres.2013.01.011 [doi]
PST - ppublish
SO  - Virus Res. 2013 May;173(2):321-6. doi: 10.1016/j.virusres.2013.01.011. Epub 2013 
      Jan 23.