PMID- 23353814
OWN - NLM
STAT- MEDLINE
DCOM- 20130905
LR  - 20220408
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 8
IP  - 1
DP  - 2013 Feb
TI  - In vitro evaluation of the interactions between human corneal endothelial cells 
      and extracellular matrix proteins.
PG  - 014108
LID - 10.1088/1748-6041/8/1/014108 [doi]
AB  - The corneal endothelium is the innermost cell layer of the cornea and rests on 
      Descemet's membrane consisting of various extracellular matrix (ECM) proteins 
      which can directly affect the cellular behaviors such as cell adhesion, 
      proliferation, polarity, morphogenesis and function. The objective of this study 
      was to investigate the interactions between the ECM environment and human corneal 
      endothelial cells (HCECs), with the ultimate goal to improve cell proliferation 
      and function in vitro. To evaluate the interaction of HCECs with ECM proteins, 
      cells were seeded on ECM-coated tissue culture dishes, including collagen type I 
      (COL I), collagen type IV (COL IV), fibronectin (FN), FNC coating mix (FNC) and 
      laminin (LM). Cell adhesion and proliferation of HCECs on each substratum and 
      expression of CEC markers were studied. The results showed that HCECs plated on 
      the COL I, COL IV, FN and FNC-coated plates had enhanced cell adhesion initially; 
      the number for COL I, COL IV, FN and FNC was significantly higher than the 
      control (P < 0.05). In addition, cells grown on ECM protein-coated dishes showed 
      more compact cellular morphology and CEC marker expression compared to cells 
      seeded on uncoated dishes. Collectively, our results suggest that an adequate ECM 
      protein combination can provide a long-term culture environment for HCECs for 
      corneal endothelium transplantation.
FAU - Choi, Jin San
AU  - Choi JS
AD  - Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, 
      Medical Center Boulevard, Winston-Salem, NC 27157, USA.
FAU - Kim, Eun Young
AU  - Kim EY
FAU - Kim, Min Jeong
AU  - Kim MJ
FAU - Giegengack, Matthew
AU  - Giegengack M
FAU - Khan, Faraaz A
AU  - Khan FA
FAU - Khang, Gilson
AU  - Khang G
FAU - Soker, Shay
AU  - Soker S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130125
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Integrins)
RN  - 0 (Laminin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/genetics
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coated Materials, Biocompatible/chemistry
MH  - Collagen/chemistry
MH  - Endothelial Cells/cytology/metabolism
MH  - Endothelium, Corneal/*cytology/*metabolism
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Fibronectins/chemistry
MH  - Gene Expression
MH  - Humans
MH  - Integrins/genetics
MH  - Laminin/chemistry
MH  - Materials Testing
MH  - Tissue Culture Techniques
EDAT- 2013/01/29 06:00
MHDA- 2013/09/06 06:00
CRDT- 2013/01/29 06:00
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/09/06 06:00 [medline]
AID - 10.1088/1748-6041/8/1/014108 [doi]
PST - ppublish
SO  - Biomed Mater. 2013 Feb;8(1):014108. doi: 10.1088/1748-6041/8/1/014108. Epub 2013 
      Jan 25.