PMID- 23354080
OWN - NLM
STAT- MEDLINE
DCOM- 20130716
LR  - 20231213
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 42
IP  - 3
DP  - 2013 Mar
TI  - AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in 
      human bladder cancer T24 cells.
PG  - 993-1000
LID - 10.3892/ijo.2013.1791 [doi]
AB  - Baicalin is one of the major compounds in the traditional Chinese medicinal herb 
      from Scutellaria baicalensis Georgi. We investigated the molecular mechanisms of 
      cell autophagy induced by baicalin in human bladder cancer T24 cells. Baicalin 
      inhibited cell survival as shown by MTT assay and increased cell death by trypan 
      blue exclusion assay in a concentration-dependent manner. Baicalin did not induce 
      apoptotic cell death in T24 cells by TUNEL and caspase-3 activity assay. Baicalin 
      induced the acidic vesicular organelle cell autophagy marker, manifested by 
      acridine orange (AO) and monodansylcadaverine (MDC) staining and cleavage of 
      microtubule-associated protein 1 light chain 3 (LC3). The protein expression 
      levels of the Atg 5, Atg 7, Atg 12, Beclin-1 and LC3-II were upregulated in T24 
      cells after baicalin treatment. Inhibition of autophagy by 3-methyl-adenine (an 
      inhibitor of class III phosphatidylinositol-3 kinase; 3-MA) reduced the cleavage 
      of LC3 in T24 cells after baicalin treatment. Furthermore, protein expression 
      levels of phospho-AKT (Ser473) and enzyme activity of AKT were downregulated in 
      T24 cells after baicalin treatment. In conclusion, baicalin triggered cell 
      autophagy through the AKT signaling pathway in T24 cells.
FAU - Lin, Chingju
AU  - Lin C
AD  - Department of Physiology, China Medical University, Taichung 404, Taiwan, R.O.C.
FAU - Tsai, Shih-Chang
AU  - Tsai SC
FAU - Tseng, Michael T
AU  - Tseng MT
FAU - Peng, Shu-Fen
AU  - Peng SF
FAU - Kuo, Sheng-Chu
AU  - Kuo SC
FAU - Lin, Meng-Wei
AU  - Lin MW
FAU - Hsu, Yuan-Man
AU  - Hsu YM
FAU - Lee, Miau-Rong
AU  - Lee MR
FAU - Amagaya, Sakae
AU  - Amagaya S
FAU - Huang, Wen-Wen
AU  - Huang WW
FAU - Wu, Tian-Shung
AU  - Wu TS
FAU - Yang, Jai-Sing
AU  - Yang JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130123
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (ATG12 protein, human)
RN  - 0 (ATG5 protein, human)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Autophagy-Related Protein 12)
RN  - 0 (Autophagy-Related Protein 5)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Small Ubiquitin-Related Modifier Proteins)
RN  - 347Q89U4M5 (baicalin)
RN  - 5142-23-4 (3-methyladenine)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 6.2.1.45 (ATG7 protein, human)
RN  - EC 6.2.1.45 (Autophagy-Related Protein 7)
RN  - EC 6.2.1.45 (Ubiquitin-Activating Enzymes)
RN  - F30N4O6XVV (Acridine Orange)
RN  - I9N81SC5HD (monodansylcadaverine)
RN  - JAC85A2161 (Adenine)
RN  - L90BEN6OLL (Cadaverine)
SB  - IM
MH  - Acridine Orange
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Apoptosis Regulatory Proteins/biosynthesis
MH  - Autophagy/*drug effects
MH  - Autophagy-Related Protein 12
MH  - Autophagy-Related Protein 5
MH  - Autophagy-Related Protein 7
MH  - Beclin-1
MH  - Cadaverine/analogs & derivatives
MH  - Caspase 3
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Enzyme Inhibitors/*pharmacology
MH  - Flavonoids/*pharmacology
MH  - Humans
MH  - Medicine, Chinese Traditional
MH  - Membrane Proteins/biosynthesis
MH  - Microtubule-Associated Proteins/biosynthesis/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Proto-Oncogene Proteins c-akt/biosynthesis/*metabolism
MH  - Signal Transduction/drug effects
MH  - Small Ubiquitin-Related Modifier Proteins/biosynthesis
MH  - Transglutaminases/antagonists & inhibitors
MH  - Ubiquitin-Activating Enzymes/biosynthesis
MH  - Urinary Bladder Neoplasms/*drug therapy
EDAT- 2013/01/29 06:00
MHDA- 2013/07/17 06:00
CRDT- 2013/01/29 06:00
PHST- 2012/11/08 00:00 [received]
PHST- 2012/12/28 00:00 [accepted]
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/07/17 06:00 [medline]
AID - 10.3892/ijo.2013.1791 [doi]
PST - ppublish
SO  - Int J Oncol. 2013 Mar;42(3):993-1000. doi: 10.3892/ijo.2013.1791. Epub 2013 Jan 
      23.