PMID- 23354096
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20161125
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 154
IP  - 3
DP  - 2013 Mar
TI  - Ontogeny of the adrenal gland in the spiny mouse, with particular reference to 
      production of the steroids cortisol and dehydroepiandrosterone.
PG  - 1190-201
LID - 10.1210/en.2012-1953 [doi]
AB  - Synthesis of the androgen dehydroepiandrosterone (DHEA) by the fetal adrenal 
      gland is important for placental estrogen production and may also be important 
      for modulating the effects of glucocorticoids on the developing brain. The 
      presence of cortisol in spiny mouse (Acomys cahirinus) blood led us to determine 
      whether the adrenal gland of this precocial rodent also synthesized DHEA. 
      Cytochrome P450 enzyme 17alpha-hydroxylase/17,20-lyase (P450c17), cytochrome-b5 
      (Cytb5), and 3beta-hydroxysteroid dehydrogenase (3betaHSD) were detected in the adrenal 
      gland from 30 days gestation (term = 39 days), and DHEA, cortisol, and 
      aldosterone were detected in fetal plasma from this time. Plasma DHEA 
      concentrations increased 4-fold, whereas cortisol concentrations decreased from 
      day 30 of gestation until the day of birth. Explant culture of fetal adrenal 
      tissue showed that DHEA was produced from exogenous pregnenolone, and thus, the 
      DHEA in the fetal circulation is likely to be of fetal origin. Clear zonation of 
      the fetal adrenal cortex was evident by 38 days gestation when expression of 
      Cytb5 was present throughout the cortex, and coexpression of P450c17 and Cytb5 
      occurred in the zona reticularis and fasciculata. 3betaHSD was expressed in the 
      cortex from at least 30 days gestation and decreased as term approached, 
      consistent with the fall of cortisol in late gestation in this species. These 
      results show that the spiny mouse adrenal gland, like that of the human fetus, 
      can synthesize and secrete DHEA from at least 30 days (relative gestation length, 
      30 days of a 39-day gestation, 0.76) of gestation, and DHEA may have important 
      roles in placental biosynthesis of estrogens and in modulating the actions of 
      glucocorticoids in the developing brain in this species.
FAU - Quinn, Tracey A
AU  - Quinn TA
AD  - The Ritchie Centre, Monash Institute of Medical Research, 27-31 Wright Street, 
      Clayton, Melbourne, Victoria, Australia 3168.
FAU - Ratnayake, Udani
AU  - Ratnayake U
FAU - Dickinson, Hayley
AU  - Dickinson H
FAU - Nguyen, Tri-Hung
AU  - Nguyen TH
FAU - McIntosh, Michelle
AU  - McIntosh M
FAU - Castillo-Melendez, Margie
AU  - Castillo-Melendez M
FAU - Conley, Alan J
AU  - Conley AJ
FAU - Walker, David W
AU  - Walker DW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130125
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - 9035-39-6 (Cytochromes b5)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/metabolism
MH  - Adrenal Glands/*embryology/growth & development/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Cytochromes b5/metabolism
MH  - Dehydroepiandrosterone/*biosynthesis/blood
MH  - Female
MH  - Fetal Blood/metabolism
MH  - Gestational Age
MH  - Humans
MH  - Hydrocortisone/*biosynthesis/blood
MH  - Immunohistochemistry
MH  - Male
MH  - Murinae/blood/*embryology/*metabolism
MH  - Pregnancy
MH  - Steroid 17-alpha-Hydroxylase/metabolism
EDAT- 2013/01/29 06:00
MHDA- 2013/04/20 06:00
CRDT- 2013/01/29 06:00
PHST- 2013/01/29 06:00 [entrez]
PHST- 2013/01/29 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
AID - en.2012-1953 [pii]
AID - 10.1210/en.2012-1953 [doi]
PST - ppublish
SO  - Endocrinology. 2013 Mar;154(3):1190-201. doi: 10.1210/en.2012-1953. Epub 2013 Jan 
      25.